RT info:eu-repo/semantics/article
T1 Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline
A1 Cobos Román, Rebeca
A1 Barreiro Méndez, Carlos
A1 Mateos, Rosa María
A1 Rubio Coque, Juan José
A2 Microbiologia
K1 Biología
K1 Biotecnología
K1 Grapevine trunck diseases
K1 Diplodia seriata
K1 Proteomics
K1 2414.06 Hongos
K1 2415 Biología Molecular
K1 2417.09 Fitopatología
AB [EN] Background: The phytopathogenic fungus Diplodia seriata, whose genome remains unsequenced, produces severe infections in fruit trees (fruit blight) and grapevines. In this crop is recognized as one of the most prominent pathogens involved in grapevine trunk disease (or grapevine decline). This pathology can result in the death of adult plants and therefore it produces severe economical losses all around the world. To date no genes or proteins have been characterized in D. seriata that are involved in the pathogenicity process. In an effort to help identify potential gene products associated with pathogenicity and to gain a better understanding of the biology of D. seriata, we initiated a proteome-level study of the fungal mycelia and secretome. Results: Intracellular and secreted proteins from D. seriata collected from liquid cultures were separated using twodimensional gel electrophoresis. About 550 cytoplasmic proteins were reproducibly present in 3 independent extractions, being 53 identified by peptide mass fingerprinting and tandem mass spectrometry. The secretome analysis showed 75 secreted proteins reproducibly present in 3 biological replicates, being 16 identified. Several of the proteins had been previously identified as virulence factors in other fungal strains, although their contribution to pathogenicity in D. seriata remained to be analyzed. When D. seriata was grown in a medium supplemented with carboxymethylcellulose, 3 proteins were up-regulated and 30 down-regulated. Within the up-regulated proteins, two were identified as alcohol dehydrogenase and mitochondrial peroxyrredoxin-1, suggesting that they could play a significant role in the pathogenicity process. As for the 30 down-regulated proteins, 9 were identified being several of them involved in carbohydrate metabolism. Conclusions: This study is the first report on proteomics on D. seriata. The proteomic data obtained will be important to understand the pathogenicity process. In fact, several of the identified proteins have been reported as pathogenicity factors in other phytopathogenic fungi. Moreover, this proteomic analysis supposes a useful basis for deepening into D. seriata knowledge and will contribute to the development of the molecular biology of this fungal strain as it has been demonstrated by cloning the gene Prx1 encoding mitochondrial peroxiredoxin-1 of D. seriata (the first gene to be cloned in this microorganism; data not shown)
PB Springer
LK https://hdl.handle.net/10612/17823
UL https://hdl.handle.net/10612/17823
NO Cobos, R., Barreiro, C., Mateos, R. M., & Coque, J. J. R. (2010). Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: A phytopathogenic fungus involved in grapevine decline. Proteome Science, 8, Article e46. https://doi.org/10.1186/1477-5956-8-46
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024