RT info:eu-repo/semantics/article
T1 Phosphate control of phoA, phoC and phoD gene expression in Streptomyces coelicolor reveals significant differences in binding of PhoP to their promoter regions
A1 Apel, Alexander Kristian
A1 Sola Landa, Alberto
A1 Rodríguez García, Antonio
A1 Martín Martín, Juan Francisco
A2 Microbiologia
K1 Biología
K1 Phosphate regulation
K1 Streptomyces
K1 Alkaline phosphatase
K1 phoA
K1 phoC
K1 phoD
K1 EMSA
K1 2415.01 Biología Molecular de Microorganismos
K1 2414.02 Fisiología Bacteriana
AB [EN] Three putative alkaline phosphatase genes, phoA, phoC and phoD, were identified in the genome of Streptomyces coelicolor by homology with the amino acid sequence obtained from the PhoA protein of Streptomyces griseus. PhoA and PhoC correspond to broad-spectrum alkaline phosphatases whereas PhoD is similar to a Ca2+-dependent phospholipase D of Streptomyces chromofuscus. The phoA and phoD genes were efficiently expressed in R5 medium under phosphate-limited conditions, as shown by studies using the xylE reporter gene, whereas phoC was poorly transcribed under the same conditions. Expression of phoA was clearly PhoP-dependent since it was not transcribed in the S. coelicolor ΔphoP mutant and was strongly activated under low phosphate concentrations. Similarly, expression of phoD was PhoP-dependent and highly sensitive to phosphate availability. By contrast, expression of phoC was not PhoP-dependent. Electrophoretic mobility shift assays showed that PhoP binds to the phoA and phoD promoters, but not to that of phoC. Footprinting studies with GST-PhoP revealed the presence of a PHO box (two direct 11 nt repeats) in the phoA promoter and two PHO boxes in the promoter of phoD. The transcription start points of the three promoters were identified by primer extension. The transcription start point of phoD coincides with the G of its translation start codon, indicating that this gene is transcribed as a leaderless mRNA. The deduced -10 and -35 regions of phoD (but not those of phoA) overlapped with the PHO boxes in this promoter, suggesting that an excess of PhoP interferes with binding of the RNA polymerase to this promoter. In summary, the three promoters showed clear differences in the modulation of their expression by PhoP
PB Microbiology Society
SN 1350-0872
LK https://hdl.handle.net/10612/17887
UL https://hdl.handle.net/10612/17887
NO Apel, A. K., Sola-Landa, A., Rodríguez-García, A., & Martín, J. F. (2007). Phosphate control of phoA, phoC and phoD gene expression in Streptomyces coelicolor reveals significant differences in binding of PhoP to their promoter regions. Microbiology, 153(10), 3527-3537. https://doi.org/10.1099/MIC.0.2007/007070-0
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 30-may-2024