RT info:eu-repo/semantics/article
T1 Target genes and structure of the direct repeats in the DNA-binding sequences of the response regulator PhoP in Streptomyces coelicolor
A1 Sola Landa, Alberto
A1 Rodríguez García, Antonio
A1 Apel, Alexander Kristian
A1 Martín Martín, Juan Francisco
A2 Microbiologia
K1 Biología
K1 Phosphate regulation
K1 PhoR-PhoP
K1 PHO boxes
K1 Streptomyces
K1 Streptomyces coelicolor
K1 EMSA
K1 DNase footprinting
K1 2415.01 Biología Molecular de Microorganismos
K1 2414.02 Fisiología Bacteriana
AB [EN] Expression of genes belonging to the pho regulon in Streptomyces coelicolor is positively regulated (as shown by comparing the wild-type and a Δ phoP mutant) by binding of the response regulator PhoP to 11-nt direct repeats (DRus). These sequences have been found in over 100 genes of Streptomyces coelicolor ; 20 of them were cloned and the binding of PhoP DBD to most of their promoters has been shown by electrophoretic mobility shift assays. Deletion experiments showed that at least two DRus are required for proper binding of PhoP DBD . Deletion of 1 nt leaving a 10-nt direct repeat reduced drastically binding of PhoP DBD . Three different types of operators have been identified. Complex operators (class III) contain up to six DRus, some of them with poor conservation of the 11-nt consensus sequence, which however were protected by PhoP DBD in footprinting analyses. A cooperative binding of PhoP DBD molecules initiated at conserved core DRus appears to be the mechanism involved in binding of several PhoP DBD monomers to those complex operators. The information theory-based model that incorporates the positive or negative contribution to the binding of PhoP DBD of adjacent sequences has been used to deduce the structure of PHO boxes and the relevance of each DRu
PB Oxford University Press
SN 0305-1048
LK https://hdl.handle.net/10612/17894
UL https://hdl.handle.net/10612/17894
NO Sola-Landa, A., Rodríguez-García, A., Apel, A. K., & Martín, J. F. (2008). Target genes and structure of the direct repeats in the DNA-binding sequences of the response regulator PhoP in Streptomyces coelicolor. Nucleic Acids Research, 36(4), 1358-1368. https://doi.org/10.1093/NAR/GKM1150
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-jun-2024