RT info:eu-repo/semantics/article
T1 Phosphate control over nitrogen metabolism in Streptomyces coelicolor: direct and indirect negative control of glnR, glnA, glnII and amtB expression by the response regulator PhoP
A1 Rodríguez García, Antonio
A1 Sola Landa, Alberto
A1 Apel, Alexander Kristian
A1 Santos Beneit, Fernando
A1 Martín Martín, Juan Francisco
A2 Microbiologia
K1 Biología
K1 Phosphate regulation
K1 Nitrogen regulation
K1 Streptomyces coelicolor
K1 GlnR
K1 PhoR-PhoP
K1 PHO boxes
K1 EMSA
K1 DNase footprinting
K1 glnA
K1 glnII
K1 amtB
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] Bacterial growth requires equilibrated concentration of C, N and P sources. This work shows a phosphate control over the nitrogen metabolism in the model actinomycete Streptomyces coelicolor. Phosphate control of metabolism in Streptomyces is exerted by the two component system PhoR-PhoP. The response regulator PhoP binds to well-known PHO boxescomposed of direct repeat units (DRus). PhoP binds to the glnR promoter, encoding the major nitrogen regulator as shown by EMSA studies, but not to the glnRII promoter under identical experimental conditions. PhoP also binds to the promoters of glnA and glnII encoding two glutamine synthetases, and to the promoter of the amtB - glnK - glnD operon, encoding an ammonium transporter and two putative nitrogen sensing/regulatory proteins. Footprinting analyses revealed that the PhoP-binding sequence overlaps the GlnR boxes in both glnA and glnII. 'Information theory' quantitative analyses of base conservation allowed us to establish the structure of the PhoP-binding regions in the glnR, glnA, glnII and amtB genes. Expression studies using luxAB as reporter showed that PhoP represses the above mentioned nitrogen metabolism genes. A mutant deleted in PhoP showed increased expression of the nitrogen metabolism genes. The possible conservation of phosphate control over nitrogen metabolism in other microorganisms is discussed
PB Oxford University Press
SN 0305-1048
LK https://hdl.handle.net/10612/17921
UL https://hdl.handle.net/10612/17921
NO Rodríguez-García, A., Sola-landa, A., Apel, K., Santos-Beneit, F., & Martín, J. F. (2009). Phosphate control over nitrogen metabolism in Streptomyces coelicolor: Direct and indirect negative control of glnR, glnA, glnII and amtB expression by the response regulator PhoP. Nucleic Acids Research, 37(10), 3230-3242. https://doi.org/10.1093/NAR/GKP162
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-jun-2024