RT info:eu-repo/semantics/article
T1 In vitro Assessment of Antiviral Effect of Natural Compounds on Porcine Epidemic Diarrhea Coronavirus
T2 Evaluación in vitro del efecto antiviral de compuestos naturales sobre el coronavirus de la diarrea epidémica porcina
A1 Gómez García, Manuel
A1 Puente Fernández, Héctor
A1 Argüello Rodríguez, Héctor
A1 Mencía Ares, Óscar
A1 Rubio Nistal, Pedro Miguel
A1 Carvajal Urueña, Ana María
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Pig
K1 Organic acids
K1 Single essential oil compounds
K1 Antiviral activity
K1 Cytopathic effect
K1 3109 Ciencias Veterinarias
K1 3104 Producción Animal
AB [EN] Organic acid and essential oils (EOs), well-known antimicrobials, could also possess antiviral activity, a characteristic which has not been completely addressed up to now. In this study, the effect of two organic acids (formic acid and sodium salt of coconut fatty acid distillates) and two single EO compounds (thymol and cinnamaldehye) was evaluated against porcine epidemic diarrhea virus (PEDV). The concentration used for each compound was established by cytotoxicity assays in Vero cells. The antiviral activity was then evaluated at three multiplicities of infection (MOIs) through visual cytopathic effect (CPE) evaluation and an alamarBlue assay as well as real-time reverse-transcription PCR (RT-qPCR) and viral titration of cell supernatants. Formic acid at at a dose of 1,200 ppm was the only compound which showed antiviral activity, with a weak reduction of CPE caused by PEDV. Through the alamarBlue fluorescence assay, we showed a significant anti-CPE effect of formic acid which could not be observed by using an inverted optical microscope. RT-qPCR and infectivity analysis also showed that formic acid significantly reduced viral RNA and viral titers in a PEDV MOI-dependent manner. Our results suggest that the antiviral activity of formic acid could be associated to its inhibitory effect on viral replication. Further studies are required to explore the anti-PEDV activity of formic acid under field conditions alone or together with other antiviral agents.
PB Frontiers Media SA
SN 2297-1769
LK https://hdl.handle.net/10612/17954
UL https://hdl.handle.net/10612/17954
NO Gómez-García, M., Puente, H., Argüello, H., Mencía-Ares, Ó., Rubio, P., & Carvajal, A. (2021). In vitro Assessment of Antiviral Effect of Natural Compounds on Porcine Epidemic Diarrhea Coronavirus. Frontiers in Veterinary Science, 8. https://doi.org/10.3389/FVETS.2021.652000
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-may-2024