RT info:eu-repo/semantics/article
T1 Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
A1 Sola Landa, Alberto
A1 Rodríguez García, Antonio
A1 Amin, Rafat
A1 Wohlleben, Wolfgang
A1 Martín Martín, Juan Francisco
A2 Microbiologia
K1 Biología
K1 Phosphate regulation
K1 Nitrogen regulation
K1 Streptomyces coelicolor
K1 PhoR-PhoP
K1 GlnR
K1 PHO boxes
K1 EMSA
K1 DNase footprinting
K1 2415.01 Biología Molecular de Microorganismos
K1 2414.02 Fisiología Bacteriana
AB [EN] Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Transcriptomic and biochemical analysis revealed that glnA (encoding a glutamine synthetase), glnR and other nitrogen metabolism genes are under PhoP control. DNA-binding experiments showed that PhoP binds to other nitrogen-regulated genes (SCO0255, SCO01863 and ureA). Using the glnA promoter as model, we observed that PhoP and GlnR compete for binding to the same promoter region, showing GlnR a higher affinity. Using a total of 14 GlnR-binding sites (50 direct repeat units) we established two information-based models that describe the GlnR box as consisting of two 11-nt direct repeats each with clear differences to PHO box. DNA-binding studies with different mutant sequences of glnA promoter revealed that the sequence recognized by GlnR is found in the coding strand whereas that recognized by PhoP is overlapping in the non-coding strand. In amtB promoter PhoP and GlnR boxes are not totally overlapping and both proteins bind simultaneously. PhoP control of nitrogen metabolism genes helps to balance the cellular P/N equilibrium
PB Oxford University Press
SN 0305-1048
LK https://hdl.handle.net/10612/17970
UL https://hdl.handle.net/10612/17970
NO Sola-Landa, A., Rodríguez-García, A., Amin, R., Wohlleben, W., & Martín, J. F. (2013). Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor. Nucleic Acids Research, 41(3), 1767-1782. https://doi.org/10.1093/NAR/GKS1203
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-jun-2024