RT info:eu-repo/semantics/article
T1 ArgR of Streptomyces coelicolor is a pleiotropic transcriptional regulator: effect on the transcriptome, antibiotic production, and differentiation in liquid cultures
A1 Botas Muñoz, Alma María
A1 Pérez Redondo, Rosario
A1 Rodríguez García, Antonio
A1 Álvarez Álvarez, Rubén
A1 Yagüe Menéndez, Paula
A1 Manteca Fernández, Ángel
A1 Liras Padín, Paloma
A2 Microbiologia
K1 Biología
K1 Streptomyces coelicolor
K1 Morphological differentiation
K1 Antibiotics biosynthesis regulation
K1 Transcriptomics
K1 ArgR
K1 Arginine regulation
K1 2415.01 Biología Molecular de Microorganismos
K1 2414.01 Antibióticos
K1 2414.02 Fisiología Bacteriana
AB [EN] ArgR is a well-characterized transcriptional repressor controlling the expression of arginine and pyrimidine biosynthetic genes in bacteria. In this work, the biological role of Streptomyces coelicolor ArgR was analyzed by comparing the transcriptomes of S. coelicolor ΔargR and its parental strain, S. coelicolor M145, at five different times over a 66-h period. The effect of S. coelicolor ArgR was more widespread than that of the orthologous protein of Escherichia coli, affecting the expression of 1544 genes along the microarray time series. This S. coelicolor regulator repressed the expression of arginine and pyrimidine biosynthetic genes, but it also modulated the expression of genes not previously described to be regulated by ArgR: genes involved in nitrogen metabolism and nitrate utilization; the act, red, and cpk genes for antibiotic production; genes for the synthesis of the osmotic stress protector ectoine; genes related to hydrophobic cover formation and sporulation (chaplins, rodlins, ramR, and whi genes); all the cwg genes encoding proteins for glycan cell wall biosynthesis; and genes involved in gas vesicle formation. Many of these genes contain ARG boxes for ArgR binding. ArgR binding to seven new ARG boxes, located upstream or near the ectA-ectB, afsS, afsR, glnR, and redH genes, was tested by DNA band-shift assays. These data and those of previously assayed fragments permitted the construction of an improved model of the ArgR binding site. Interestingly, the overexpression of sporulation genes observed in the ΔargR mutant in our culture conditions correlated with a sporulation-like process, an uncommon phenotype
PB Frontiers Media SA
LK https://hdl.handle.net/10612/18003
UL https://hdl.handle.net/10612/18003
NO Botas, A., Pérez-Redondo, R., Rodríguez-García, A., Álvarez-álvarez, R., Yagüe, P., Manteca, A., & Liras, P. (2018). ArgR of Streptomyces coelicolor is a pleiotropic transcriptional regulator: Effect on the transcriptome, antibiotic production, and differentiation in liquid cultures. Frontiers in Microbiology, 9, Article e361. https://doi.org/10.3389/FMICB.2018.00361
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 15-may-2024