RT info:eu-repo/semantics/article
T1 Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions
A1 Fernández Alegre, Estela
A1 Alvarez-Fernandez, Indira
A1 Domínguez Fernández de Tejerina, Juan Carlos
A1 Casao, Adriana
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Biología
K1 Melatonin
K1 Spermatozoon
K1 Bull
K1 Sperm motility
K1 Sperm physiology
K1 3104.11 Reproducción
AB [EN] Bull spermatozoa physiology may be modulated by melatonin. We washed ejaculated spermatozoa free of melatonin and incubated them (4 h, 38 °C) with 0-pM, 1-pM, 100-pM, 10-nM and 1-µM melatonin in TALP-HEPES (non-capacitating) and TALP-HEPES-heparin (capacitating). This range of concentrations encompassed the effects mediated by melatonin receptors (pM), intracellular targets (nM–µM) or antioxidant activity (µM). Treatment effects were assessed as motility changes by computer-assisted sperm analysis (CASA) of motility and physiological changes by flow cytometry. Melatonin effects were more evident in capacitating conditions, with 100 pM reducing motility and velocity (VCL) while increasing a “slow” subpopulation. All concentrations decreased apoptotic spermatozoa and stimulated mitochondrial activity in viable spermatozoa, with 100 pM–1 µM increasing acrosomal damage, 10 nM–1 µM increasing intracellular calcium and 1 pM reducing the response to a calcium-ionophore challenge. In non-capacitating media, 1 µM increased hyperactivation-related variables and decreased apoptotic spermatozoa; 100 pM–1 µM increased membrane disorders (related to capacitation); all concentrations decreased mitochondrial ROS production. Melatonin concentrations had a modal effect on bull spermatozoa, suggesting a capacitation-modulating role and protective effect at physiological concentrations (pM). Some effects may be of practical use, considering artificial reproductive techniques
PB MDPI
LK https://hdl.handle.net/10612/18004
UL https://hdl.handle.net/10612/18004
NO Fernández-Alegre, E., Álvarez-Fernández, I., Domínguez, J. C., Casao, A., & Martínez-Pastor, F. (2020). Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions. International Journal of Molecular Sciences, 21(8), Article e2701. https://doi.org/10.3390/ijms21082701
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 13-may-2024