RT info:eu-repo/semantics/article
T1 A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms
A1 De Souza Fonseca, Pablo Augusto
A1 Rosse, I.C.
A1 DeMiranda, M.
A1 Machado, M.A.
A1 Verneque, R.S.
A1 Diniz Peixoto, Maria Gabriela Campolina
A1 Santos Carvalho, Maria Raquel
A2 Genetica
K1 Genética
K1 CSN3
K1 Tetra-primer ARMS-PCR
K1 Casein
K1 Polymorphism
K1 K-casein
K1 3109.02 Genética
AB [EN] Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduces the loss of milk solids, enhancing cheese yield. This polymorphism induces a double amino acid substitution (Thr136Ile and Ala148Asp). The polymorphism is normally detected by PCR-RFLP, which is a laborious method. An interesting methodological alternative is the tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR). A tetra-primer ARMS-PCR for the detection of this κ-casein polymorphism has been described. However, specificity was not achieved, probably due to problems with primer design. We developed a new tetra-primer ARMS-PCR for the detection of the κ-casein polymorphism. This new method was validated in a double-blind test, by comparison with the results obtained for 50 Guzerá bulls formerly genotyped by PCR-RFLP. This new method achieved 100% sensitivity and specificity. We conclude that this method is a useful, cost-efficient alternative for the detection of functional κ-casein polymorphisms
PB FUNPEC-RP
LK https://hdl.handle.net/10612/18022
UL https://hdl.handle.net/10612/18022
NO Fonseca, P. A. S., Rosse, I. C., DeMiranda, M., Machado, M. A., Verneque, R. S., Peixoto, M. G. C. D., & Carvalho, M. R. S. (2013). A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms. Genet Mol Res, 12(4), 6521-6526.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 14-may-2024