RT info:eu-repo/semantics/article
T1 Manganese transporter protein MntH is required for virulence of Xylophilus ampelinus, the causal agent of bacterial necrosis in grapevine
A1 Sevillano Nistal, Silvia
A1 Cobos Román, Rebeca
A1 García Angulo, Penélope
A1 Alonso Monroy, Alberto
A1 Álvarez Rodríguez, María Luisa
A1 Álvarez Pérez, José Manuel
A1 Olego Morán, Miguel Ángel
A1 Acebes Arranz, José Luis
A1 Garzón Jimeno, José Enrique
A1 Rubio Coque, Juan José
A2 Microbiologia
K1 Biología
K1 Biotecnología
K1 MntH protein
K1 Bacterial necrosis
K1 Grapevine
K1 Virulence
K1 Xylophilus ampelinus
K1 2417.09 Fitopatología
K1 3108.01 Bacterias
K1 3108.08 Susceptibilidad y Resistencia Vegetal
AB [EN] Background and Aims: The aim of this study is to identify proteins involved in the pathogenicity/virulence of Xylophilus ampelinus. Characterisation of these proteins could provide new insights into putative targets for controlling bacterial necrosis in grapevines. Methods and Results: Transposon insertion mutagenesis was used to isolate X. ampelinus mutants exhibiting an altered virulence. Characterisation of one of the avirulent mutants revealed the insertion of a transposon into the mntH gene encoding the major manganese transporter. Virulence tests on grapevine leaves clearly showed that the virulence of these mutants was significantly reduced. Phenotypic analysis of an mntH mutant indicated that the MntH protein is a Mn++ transporter but that MntH does not play a significant role in the transport of Fe++ or Cu++. The MntH mutants exhibited an increased sensitivity to hydrogen peroxide, although catalase and superoxide dismutase activities were not significantly affected. Conclusion: The MntH protein plays a significant role in the virulence of X. ampelinus. Significance of the Study: This is the first report showing that transposon mutagenesis is an effective strategy for the isolation of X. ampelinus mutants. It is also the first report characterising a gene encoding a protein involved in virulence in this grapevine pathogen
PB Hindawi
SN 1322-7130
LK https://hdl.handle.net/10612/18033
UL https://hdl.handle.net/10612/18033
NO Sevillano, Cobos, García-Angulo, Alonso-Monroy, Álvarez-Rodríguez, Álvarez-Pérez, Olego, Acebes, Garzón, & Coque, J. J. R. (2014). Manganese transporter protein MntH is required for virulence of Xylophilus ampelinus, the causal agent of bacterial necrosis in grapevine. Australian Journal of Grape and Wine Research, 20(3), 442-450. https://doi.org/10.1111/AJGW.12090
NO This journal is published by Hindawi as part of a publishing collaboration with the Australian Society of Viticulture and Oenology. It is a fully open access journal produced under the Hindawi and Wiley brands
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 22-may-2024