RT info:eu-repo/semantics/article
T1 Type III antifreeze protein (AFP) improves the post-thaw quality and in vivo fertility of rooster spermatozoa
A1 Mehdipour, Mahdieh
A1 Daghigh Kia, Hossein
A1 Najafi, Abouzar
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Biología
K1 Type III antifreeze protein
K1 Rooster
K1 Spermatozoon
K1 Cryopreservation
K1 3104.11 Reproducción
AB [EN] Antifreeze proteins (AFP) have the potential for improving sperm cryopreservation. We have applied Type III antifreeze protein (AFP3) on the cryopreservation of spermatozoa from broiler breeder roosters, aiming to enhance post-thawing quality and fertility. Semen was extended at 37°C in Lake's extender containing AFP3 at 0.01, 0.1, 1, 5, and 10 µg/mL (no AFP3 as control). Post-thawing sperm assessment included sperm motility (CASA), morphology, membrane functionality by hypoosmotic swelling test (HOST), lipoperoxidation as malondialdehyde (MDA) production, and sperm viability, early apoptosis (phosphatidylserine exposure as annexin V-positive staining in viable spermatozoa), and mitochondrial activity by flow cytometry. Fertility was assessed after artificial insemination (30 hens/treatment). Total and progressive motility, membrane functionality, and mitochondrial activity increased in 0.1 and 1 µg/mL AFP, compared to control and other concentrations, whereas apoptosis was significantly lower. VAP, VSL, and viability were significantly higher for 1 µg/mL AFP3 than with the other treatments except for 0.1 µg/mL (which was not always significantly different from the control or other concentrations), and with abnormal forms being significantly lower. The proportion of fertilized and hatched eggs was also higher for 1 µg/mL AFP3, with 0.1 µg/mL also showing significantly higher results than the control, and no differences with other concentrations). In conclusion, 1 µg/mL AFP3 could improve the post-thawing results of rooster spermatozoa frozen in Lake's extender. According to our results, concentrations between 1 and 0.1 µg/mL could be similarly efficient
PB Elsevier
SN 0032-5791
LK https://hdl.handle.net/10612/18076
UL https://hdl.handle.net/10612/18076
NO Mehdipour, M., Daghigh-Kia, H., Najafi, A., & Martínez-Pastor, F. (2021). Type III antifreeze protein (AFP) improves the post-thaw quality and in vivo fertility of rooster spermatozoa. Poultry Science, 100(8), Article e101291. https://doi.org/10.1016/j.psj.2021.101291
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024