RT info:eu-repo/semantics/article
T1 Single layer centrifugation (SLC) for bacterial removal with Porcicoll positively modifies chromatin structure in boar spermatozoa
A1 Lacalle Fernández, Estíbaliz
A1 Fernández Alegre, Estela
A1 Soriano Úbeda, Cristina de las Mercedes
A1 Martínez Martínez, Sonia
A1 Domínguez Fernández de Tejerina, Juan Carlos
A1 González Montaña, José Ramiro
A1 Morrell, Jane Margaret
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Biología
K1 Boar
K1 DNA fragmentation
K1 Chromatin
K1 Canonical correlation analysis
K1 Single layer centrifugation
K1 3104.11 Reproducción
AB [EN] The storage of boar semen samples at 17 °C for artificial insemination (AI) doses enables the proliferation of the bacteria, making antibiotics necessary. This can contribute to the development of antimicrobial resistance (AMR). This study tested bacterial presence and sperm chromatin structure after using a low-density colloid (Porcicoll) as an antibiotic alternative to eliminate bacteria. Ejaculates (8 boars, 3 ejaculates each) were split as control and low-density colloid centrifugation (single layer centrifugation, SLC, 20%, and 30% Porcicoll) into 500 ml tubes. Analyses were carried out at days 0, 3, and 7 (17 °C) for microbial presence and sperm chromatin structure analysis: %DFI (DNA fragmentation) and %HDS (chromatin immaturity), monobromobimane (mBBr; free thiols and disulfide bridges), and chromomycin A3 (CMA3; chromatin compaction). Besides comparing bacterial presence (7 species identified) and chromatin variables between treatments, the associations between these sets of variables were described by canonical correlation analysis (CCA). Results showed a significant decrease of some bacteria or a complete removal after SLC (especially for P30). SLC also caused a decrease of %HDS and an increase of disulfide bridges and low and medium mBBr populations, suggesting the removal of immature sperm (poor chromatin compaction). CCA showed an association pattern compatible with the degradation of sperm chromatin parameters with bacterial contamination, especially Enterobacteria, P. aeuriginosa, and K. variicola. In conclusion, bacterial contamination affects sperm chromatin beyond DNA fragmentation; SLC with low-density colloid not only removes bacteria from boar semen, but also chromatin structure is enhanced after selection
PB Elsevier
SN 0093-691X
LK https://hdl.handle.net/10612/18082
UL https://hdl.handle.net/10612/18082
NO Lacalle, E., Fernández-Alegre, E., Soriano-Úbeda, C., Martínez-Martínez, S., Domínguez, J. C., González-Montaña, J. R., Morrell, J. M., & Martínez-Pastor, F. (2023). Single layer centrifugation (SLC) for bacterial removal with Porcicoll positively modifies chromatin structure in boar spermatozoa. Theriogenology, 201, 95–105. https://doi.org/10.1016/j.theriogenology.2023.02.017
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 11, 2024