RT info:eu-repo/semantics/article
T1 Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A
A1 Del Carratore, Francesco
A1 Iorio, Marianna
A1 Pérez-Bonilla, Mercedes
A1 Schmidt, Kamila
A1 Pérez Redondo, Rosario
A1 Sosio, Margherita
A1 Macdonald, Sandy J.
A1 Gyulev, Ivan S.
A1 Tsigkinopoulou, Areti
A1 Thomas, Gavin H.
A1 Genilloud, Olga
A1 Rodríguez García, Antonio
A1 Donadio, Stefano
A1 Breitling, Rainer
A1 Takano, Eriko
A2 Microbiologia
K1 Biología
K1 Biotecnología
K1 Planobispora rosea
K1 Antibiotics biosynthesis regulation
K1 Phosphate regulation
K1 Transcriptomics
K1 Metabolomics
K1 Systems biology
K1 Thiopeptide antibiotics
K1 GE2270A
K1 Genome-scale metabolic model
K1 2414.01 Antibióticos
K1 2414.02 Fisiología Bacteriana
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] Planobispora rosea is the natural producer of the potent thiopeptide antibiotic GE2270A. Here, we present the results of a metabolomics and transcriptomics analysis of P. rosea during production of GE2270A. The data generated provides useful insights into the biology of this genetically intractable bacterium. We characterize the details of the shutdown of protein biosynthesis and the respiratory chain associated with the end of the exponential growth phase. We also provide the first description of the phosphate regulon in P. rosea. Based on the transcriptomics data, we show that both phosphate and iron are limiting P. rosea growth in our experimental conditions. Additionally, we identified and validated a new biosynthetic gene cluster associated with the production of the siderophores benarthin and dibenarthin in P. rosea. Together, the metabolomics and transcriptomics data are used to inform and refine the very first genome-scale metabolic model for P. rosea, which will be a valuable framework for the interpretation of future studies of the biology of this interesting but poorly characterized species
PB American Society for Microbiology
LK https://hdl.handle.net/10612/18086
UL https://hdl.handle.net/10612/18086
NO Del Carratore, F., Iorio, M., Pérez-Bonilla, M., Schmidt, K., Pérez-Redondo, R., Sosio, M., Macdonald, S. J., Gyulev, I. S., Tsigkinopoulou, A., Thomas, G. H., Genilloud, O., Rodríguez-García, A., Donadio, S., Breitling, R., & Takano, E. (2021). Multi-omics study of planobispora rosea, producer of the thiopeptide antibiotic GE2270A. mSystems, 6(3), Article e341. https://doi.org/10.1128/MSYSTEMS.00341-21
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024