RT info:eu-repo/semantics/article
T1 Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
A1 Lombó Alonso, Marta
A1 Herráez Ortega, María Paz
A2 Biologia Celular
K1 Biología
K1 Cardiogenesis
K1 Zebrafish
K1 Paternal transmission
K1 Epigenetics
K1 Bisphenol A
K1 Paternal exposure
K1 Histone acetylation
K1 Sperm epigenome
K1 Heart development
K1 2401.07 Embriología Animal
K1 2407 Biología Celular
K1 3214 Toxicología
K1 2409.99 Otros (Epigenética)
AB [EN] Parental exposure to bisphenol A (BPA) has been linked to a greater incidence of congenital diseases. We have demonstrated that BPA induces in zebrafish males an increase in the acetylation of sperm histones that is transmitted to the blastomeres of the unexposed progeny. This work is aimed to determine whether histone hyperacetylation promoted by paternal exposure to BPA is the molecular mechanism underlying the cardiogenesis impairment in the descendants. Zebrafish males were exposed to 100 and 2000 µg/L BPA during early spermatogenesis and mated with non-exposed females. We analyzed in the progeny the expression of genes involved in cardiogenesis and the epigenetic profile. Once the histone hyperacetylation was confirmed, treatment with epigallocatechin gallate (EGCG), an inhibitor of histone acetyltransferases, was assayed on F1 embryos. Embryos from males exposed to 2000 µg/L BPA overexpressed the transcription factor hand2 and the receptor esr2b, showing their own promoters—as well as that of kat6a—an enrichment in H3K9ac. In embryos treated with EGCG, both gene expression and histone acetylation (global and specific) returned to basal levels, and the phenotype was recovered. As shown by the results, the histone hyperacetylated landscape promoted by BPA in the sperm alters the chromatin structure of the progeny, leading to the overexpression of the histone acetyltransferase and genes involved in cardiogenesis
PB MDPI
LK https://hdl.handle.net/10612/18095
UL https://hdl.handle.net/10612/18095
NO Lombó, M., & Herráez, M. P. (2021). Paternal inheritance of bisphenol a cardiotoxic effects: The implications of sperm epigenome. International Journal of Molecular Sciences, 22(4), Article e2125. https://doi.org/10.3390/IJMS22042125
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 30-jun-2024