RT info:eu-repo/semantics/article T1 Current challenges in sheep artificial insemination: A particular insight A1 Álvarez García, Mercedes A1 Anel López, Luis A1 González Boixo, José Carlos A1 Chamorro Álvarez, César Ángel A1 Neila Montero, Marta A1 Montes Garrido, Rafael A1 Paz Cabello, Paulino de A1 Anel Rodríguez, Luis A2 Medicina y Cirugia Animal K1 Veterinaria K1 AI K1 Cervix K1 Cryopreservation K1 Ovine K1 Sperm K1 Transcervical K1 3109 Ciencias Veterinarias AB [EN] Ovine artificial insemination (OAI) is not commonly performed because of specific problems related to semen application techniques, leading to highly variable results. The ideal methodology (frozen-thawed semen/vaginal route) is unfeasible under field conditions due to the cervix morphology of the ewe, which prevents the process of intrauterine insemination necessary to obtain acceptable results. Currently, OAI commercial programmes use superficial cervical insemination, CAI (vaginal), with chilled semen (15°C) and intrauterine insemination, LAI (laparoscopic), with frozen-thawed semen. The ability to improve upon these contrasting techniques may be derived from examining certain poorly studied factors such as insemination time, productive state of females and alternatives of seminal preservation, some of which we reviewed in this work. This interim solution will remain in use until AI by the vaginal route with frozen-thawed semen is developed, but it poses new challenges in optimizing the freezing of the sperm and adapting the cervical (CAI) and/or transcervical intrauterine AI (TCAI). In this review, we address the current problems and evaluate their methodological (mechanical) and chemical (dilation) alternatives. Currently, TCAI is a methodologically complex technique with poor fertility results, so further studies are needed to improve the logistics of this procedure and the results of its application PB Wiley SN 0936-6768 LK https://hdl.handle.net/10612/18281 UL https://hdl.handle.net/10612/18281 NO Álvarez García, M., Anel-Lopez, L., Boixo, J. C., Chamorro, C., Neila-Montero, M., Montes-Garrido, R., de Paz, P., & Anel, L. (2019). Current challenges in sheep artificial insemination: A particular insight. Reproduction in Domestic Animals, 54(S4), 32-40. https://doi.org/10.1111/RDA.13523 DS BULERIA. Repositorio Institucional de la Universidad de León RD 17-may-2024