RT info:eu-repo/semantics/article
T1 Sperm Cell Population Dynamics in Ram Semen during the Cryopreservation Process
A1 Ramón Fernández, Manuel
A1 Pérez Guzmán, María Dolores
A1 Jiménez Rabadán, Pilar
A1 Esteso Díaz, Milagros Cristina
A1 García Álvarez, Olga
A1 Maroto Morales, Alejandro
A1 Anel López, Luis
A1 Soler Valls, Ana Josefa
A1 Fernández Santos, María Rocío
A1 Garde López-Brea, Julián
A2 Sanidad Animal
K1 Veterinaria
K1 Sperm
K1 Cell population
K1 Ram
K1 Semen
K1 Cryopreservation
AB [EN] Background: Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols in different species that would allow for an efficient storage of semen samples for long periods of time, ensuring its viability. Nowadays, it is widely known that an important individual component exists in the cryoresistance of semen, and efforts are aimed at identifying those sperm characteristics that may allow us to predict this cryoresistance. This knowledge would lead, ultimately, to the design of optimized freezing protocols for the sperm characteristics of each male. Methodology/Principal Findings: We have evaluated the changes that occur in the sperm head dimensions throughout the cryopreservation process. We have found three different patterns of response, each of one related to a different sperm quality at thawing. We have been able to characterize males based on these patterns. For each male, its pattern remained constant among different ejaculates. This latter would imply that males always respond in the same way to freezing, giving even more importance to this sperm feature. Conclusions/Significance: Changes in the sperm head during cryopreservation process have resulted useful to identify the ability of semen of males for freezing. We suggest that analyses of these response patterns would represent an important tool to characterize the cryoresistance of males when implemented within breeding programs. We also propose follow-up experiments to examine the outcomes of the use of different freezing protocols depending on the pattern of response of males.
PB Public Library of Science
LK https://hdl.handle.net/10612/18514
UL https://hdl.handle.net/10612/18514
NO Ramón, M., Pérez-Guzmán, M. D., Jiménez-Rabadán, P., Esteso, M. C., García-Álvarez, O., Maroto-Morales, A., Anel-López, L., Soler, A. J., Fernández-Santos, M. R., & Garde, J. J. (2013). Sperm Cell Population Dynamics in Ram Semen during the Cryopreservation Process. PLoS ONE, 8(3). https://doi.org/10.1371/JOURNAL.PONE.0059189
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 14-may-2024