RT info:eu-repo/semantics/article
T1 Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
A1 Fernández Veiga, Leire
A1 Fuertes Franco, Miguel
A1 Geijo, María V.
A1 Pérez de Val, Bernat
A1 Vidal, Enric
A1 Michelet, Lorraine
A1 Boschiroli, María Laura
A1 Gómez Buendía, Alberto
A1 Bezos, Javier
A1 Jones, Gareth J.
A1 Vordermeier, Martin
A1 Juste, Ramón A.
A1 Garrido, Joseba M.
A1 Sevilla, Iker A.
A2 Sanidad Animal
K1 Sanidad animal
K1 Mycobacterium
K1 Tuberculosis
K1 Antigens
K1 3109 Ciencias Veterinarias
AB [EN] The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity
PB Nature research
LK https://hdl.handle.net/10612/18585
UL https://hdl.handle.net/10612/18585
NO Fernández-Veiga, L., Fuertes, M., Geijo, M. V., Pérez de Val, B., Vidal, E., Michelet, L., Boschiroli, M. L., Gómez-Buendía, A., Bezos, J., Jones, G. J., Vordermeier, M., Juste, R. A., Garrido, J. M., & Sevilla, I. A. (2023). Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria. Scientific Reports, 13(1). https://doi.org/10.1038/S41598-023-30147-4
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024