RT info:eu-repo/semantics/article
T1 Limited Probiotic Effect of Enterococcus gallinarum L1, Vagococcus fluvialis L21 and Lactobacillus plantarum CLFP3 to Protect Rainbow Trout against Saprolegniosis
A1 Fregeneda Grandes, Juan Miguel
A1 González Palacios, Concepción
A1 Pérez Sánchez, Tania
A1 Padilla, Daniel
A1 Real, Fernando
A1 Aller Gancedo, José Miguel
A2 Sanidad Animal
K1 Veterinaria
K1 Biocontrol
K1 Probiotics
K1 Saprolegniosis
K1 Inhibition assays
K1 Oncorhynchus mykiss
K1 3109 Ciencias Veterinarias
AB [EN] Previous studies have demonstrated that the strains Enterococcus gallinarum L1, Vagococcus fluvialis L21 and Lactobacillus plantarum CLFP3 are probiotics against vibriosis or lactococosis in sea bass or rainbow trout. In this study, the utility of these bacterial strains in the control of saprolegniosis was evaluated. For this purpose, both in vitro inhibition studies and competition for binding sites against Saprolegnia parasitica and in vivo tests with experimentally infected rainbow trout were carried out. In the in vitro tests, the three isolates showed inhibitory activity upon mycelium growth and cyst germination and reduced the adhesion of cysts to cutaneous mucus; however, this effect depended on the number of bacteria used and the incubation time. In the in vivo test, the bacteria were administered orally at 108 CFU g−1 in the feed or at 106 CFU ml−1 in the tank water for 14 days. None of the three bacteria showed protection against S. parasitica infection either through water or feed, and the cumulative mortality reached 100% within 14 days post infection. The obtained results show that the use of an effective probiotic against a certain disease in a host may not be effective against another pathogen or in another host and that the results obtained in vitro may not always predict the effects when used in vivo.
PB MDPI
LK https://hdl.handle.net/10612/18599
UL https://hdl.handle.net/10612/18599
NO Fregeneda Grandes, J.-M., González-Palacios, C., Pérez-Sánchez, T., Padilla, D., Real, F., & Aller-Gancedo, J.-M. (2023). Limited Probiotic Effect of Enterococcus gallinarum L1, Vagococcus fluvialis L21 and Lactobacillus plantarum CLFP3 to Protect Rainbow Trout against Saprolegniosis. Animals, 13(5). https://doi.org/10.3390/ANI13050954
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 30-jun-2024