RT info:eu-repo/semantics/article
T1 DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids
A1 Cánovas, Sebastian
A1 Ivanova, Elena
A1 Romar, Raquel
A1 García Martínez, Soledad
A1 Soriano Úbeda, Cristina de las Mercedes
A1 García Vázquez, Francisco Alberto
A1 Saadeh, Heba
A1 Andrews, Simon
A1 Kelsey, Gavin
A1 Coy, Pilar
A2 Medicina y Cirugia Animal
K1 Sanidad animal
K1 Veterinaria
K1 Reproducción
K1 Embriones
K1 Fecundación in vitro
K1 Cerdos
K1 3109 Ciencias Veterinarias
K1 3104 Producción Animal
K1 2411.16 Fisiología de la Reproducción
AB [EN]The number of children born since the origin of Assisted Reproductive Technologies(ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has beenreported among ART-conceived infants, suggesting an epigenetic cost. We report the first wholegenome DNA methylation datasets from single pig blastocysts showing differences between in vivoand in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in thepresence of natural reproductive fluids (Natur-IVF). Natur-IVF embryos were of higher quality thanC-IVF in terms of cell number and hatching ability. RNA-Seq and DNA methylation analyses showedthat Natur-IVF embryos have expression and methylation patterns closer to in vivo blastocysts.Genes involved in reprogramming, imprinting and development were affected by culture, withfewer aberrations in Natur-IVF embryos. Methylation analysis detected methylated changes inC-IVF, but not in Natur-IVF, at genes whose methylation could be critical, such as IGF2R andNNAT.
PB eLife Sciences Publications
LK https://hdl.handle.net/10612/18601
UL https://hdl.handle.net/10612/18601
NO Canovas, S., Ivanova, E., Romar, R., García-Martínez, S., Soriano-Úbeda, C., García-Vázquez, F. A., Saadeh, H., Andrews, S., Kelsey, G., & Coy, P. (2017). DNA methylation and gene expression changes derived from assisted reproductive technologies can be decreased by reproductive fluids. eLife, 6. https://doi.org/10.7554/ELIFE.23670
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 14-may-2024