RT info:eu-repo/semantics/article
T1 Mitochondrial dysfunction promotes microbial composition that negatively impacts on ulcerative colitis development and progression
A1 Peña Cearra, Ainize
A1 Song, Deguang
A1 Castelo, Janire
A1 Palacios, Ainhoa
A1 Lavín, José Luis
A1 Azkargorta, Mikel
A1 Elortza, Félix
A1 Fuertes Franco, Miguel
A1 Pascual Itoiz, Miguel Ángel
A1 Barriales, Diego
A1 Martín Ruiz, Itziar
A1 Fullaondo, Asier
A1 Aransay, Ana M.
A1 Rodriguez, Héctor
A1 Palm, Noah W.
A1 Anguita, Juan
A1 Abecia, Leticia
A2 Sanidad Animal
K1 Medicina. Salud
K1 Sanidad animal
K1 Clinical microbiology
K1 Microbiota
K1 Colitis ulcerosa
K1 3109.05 Microbiología
AB [EN] Recent evidence demonstrates potential links between mitochondrial dysfunction and inflammatory bowel diseases (IBD). Inaddition, bidirectional interactions between the intestinal microbiota and host mitochondria may modulate intestinal inflammation.We observed previously that mice deficient in the mitochondrial protein MCJ (Methylation-controlled J protein) exhibit increasedsusceptibility to DSS colitis. However, it is unclear whether this phenotype is primarily driven by MCJ−/− associated gut microbiotadysbiosis or by direct effects of MCJ-deficiency. Here, we demonstrate that fecal microbiota transplantation (FMT) from MCJdeficient into germ-free mice was sufficient to confer increased susceptibility to colitis. Therefore, an FMT experiment by cohousingwas designed to alter MCJ-deficient microbiota. The phenotype resulting from complex I deficiency was reverted by FMT. Inaddition, we determined the protein expression pathways impacted by MCJ deficiency, providing insight into the pathophysiologyof IBD. Further, we used magnetic activated cell sorting (MACS) and 16S rRNA gene sequencing to characterize taxa-specific coatingof the intestinal microbiota with Immunoglobulin A (IgA-SEQ) in MCJ-deficient mice. We show that high IgA coating of fecalbacteria observed in MCJ-deficient mice play a potential role in disease progression. This study allowed us to identify potentialmicrobial signatures in feces associated with complex I deficiency and disease progression. This research highlights the importanceof finding microbial biomarkers, which might serve as predictors, permitting the stratification of ulcerative colitis (UC) patients intodistinct clinical entities of the UC spectrum.
PB Nature Research
LK https://hdl.handle.net/10612/18602
UL https://hdl.handle.net/10612/18602
NO Peña-Cearra, A., Song, D., Castelo, J., Palacios, A., Lavín, J. L., Azkargorta, M., Elortza, F., Fuertes, M., Pascual-Itoiz, M. A., Barriales, D., Martín-Ruiz, I., Fullaondo, A., Aransay, A. M., Rodríguez, H., Palm, N. W., Anguita, J., & Abecia, L. (2023). Mitochondrial dysfunction promotes microbial composition that negatively impacts on ulcerative colitis development and progression. npj Biofilms and Microbiomes, 9(1). https://doi.org/10.1038/S41522-023-00443-Y
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 16-jun-2024