RT info:eu-repo/semantics/article
T1 Impact of sperm DNA damage and oocyte-repairing capacity on trout development
A1 Fernández Díez, Cristina
A1 González Rojo, Silvia
A1 Lombó Alonso, Marta
A1 Herráez Ortega, María Paz
A2 Biologia Celular
K1 Biología
K1 Sperm
K1 DNA damage
K1 Oocyte repairing
K1 Trout development
K1 2407 Biología Celular
K1 3104.11 Reproducción
K1 2401.07 Embriología Animal
AB [EN] Zygotic repair of paternal DNA is essential during embryo development. In spite of the interest devoted to sperm DNA damage, its combined effect with defect-repairing oocytes has not been analyzed. Modification of the breeding season is a common practice in aquaculture. This practice reduces developmental success and could affect the both factors: sperm DNA integrity and oocyte repair capacity. To evaluate the maternal role, we analyzed the progeny outcome after fertilizing in-season trout oocytes with untreated and with UV-irradiated sperm. We also analyzed the offspring obtained out of season with untreated sperm. The analysis of the number of lesions in 4 sperm nuclear genes revealed an increase of 1.22–11.18 lesions/10 kb in out-of-season sperm, similar to that obtained after sperm UV irradiation (400 µW/cm25 min). Gene expression showed in out-of-season oocytes the overexpression of repair genes (ogg1, ung, lig3, rad1) and downregulation of tp53, indicating an enhanced repairing activity and reduced capacity to arrest development upon damage. The analysis of the progeny in out-of-season embryos revealed a similar profile tolerant to DNA damage, leading to a much lower apoptotic activity at organogenesis, lower hatching rates and increased rate of malformations. The effects were milder in descendants from in-season-irradiated sperm, showing an enhanced repairing activity at epibolia. Results point out the importance of the repairing machinery provided by the oocyte and show how susceptible it is to environmental changes. Transcripts related to DNA damage signalization and repair could be used as markers of oocyte quality
PB Society for Reproduction and Fertility
SN 1470-1626
LK https://hdl.handle.net/10612/18707
UL https://hdl.handle.net/10612/18707
NO Fernández-Díez, C., González-Rojo, S., Lombó, M., & Herráez, M. P. (2016). Impact of sperm DNA damage and oocyte-repairing capacity on trout development. Reproduction, 152(1), 57-67. https://doi.org/10.1530/REP-16-0077
NO Palabras clave extraídas del título
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 11, 2024