RT info:eu-repo/semantics/article
T1 Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis
A1 Fernández, Miguel
A1 Royo, Marcos
A1 Arteche Villasol, Noive
A1 Ferreras Estrada, María Del Carmen
A1 Benavides, Julio
A1 Pérez Pérez, Valentín
A2 Sanidad Animal
K1 Sanidad animal
K1 Paratuberculosis
K1 Vaccination
K1 Ruminants
K1 Mycobacterium avium subspecies paratuberculosis
K1 Interferon-gamma
K1 Immune response
K1 Tuberculosis
K1 Antigens
K1 3109 Ciencias Veterinarias
AB [EN] Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of Mycobacterium bovis (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of Mycobacterium avium subspecies paratuberculosis (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new Mycobacterium bovis antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats
PB MDPI
LK https://hdl.handle.net/10612/18809
UL https://hdl.handle.net/10612/18809
NO Fernández, M., Royo, M., Arteche-Villasol, N., Ferreras, M. C., Benavides, J., & Pérez, V. (2022). Peripheral IFN-ɣ Production after Blood Stimulation with Different Mycobacterial Antigens in Goats Vaccinated against Paratuberculosis. Vaccines, 10(10). https://doi.org/10.3390/VACCINES10101709
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-may-2024