RT info:eu-repo/semantics/article
T1 Bovine Intelectin 2 Expression as a Biomarker of Paratuberculosis Disease Progression
A1 Blanco Vázquez, Cristina
A1 Balseiro Morales, Ana María
A1 Alonso Hearn, Marta
A1 Juste, Ramón A.
A1 Iglesias, Natalia
A1 Canive, María
A1 Casais, Rosa
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Intelectin 2
K1 Biomarker
K1 Immunohistochemistry
K1 Ileocecal valve
K1 Mycobacterium avium subsp. paratuberculosis
K1 Paratuberculosis
K1 3109 Ciencias Veterinarias
K1 3109.05 Microbiología
K1 3104.02 Bovinos
AB [EN]Paratuberculosis (PTB), a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), is responsible for important economic losses in the dairy indus-try. Our previous RNA-sequencing (RNA-Seq) analysis showed that bovine intelectin 2 (ITLN2) precursor gene was overexpressed in ileocecal valve (ICV) samples of animals with focal (log2 fold-change = 10.6) and diffuse (log2 fold-change = 6.8) PTB-associated lesions compared to animals without lesions. This study analyzes the potential use of ITLN2, a protein that has been described as fundamental in the innate immune response to infections, as a biomarker of MAP infection. The presence of ITLN2 was investigated by quantitative immunohistochemical analysis of ICV samples of 20 Holstein Friesian cows showing focal (n = 5), multifocal (n = 5), diffuse (n = 5) and no histological lesions (n = 5). Significant differences were observed in the mean number of ITLN2 immunostained goblet and Paneth cells between the three histopathological types and the control. The number of immunolabelled cells was higher in the focal histopathological type (116.9 ± 113.9) followed by the multifocal (108.7 ± 140.5), diffuse (76.5 ± 97.8) and control types (41.0 ± 81.3). These results validate ITLN2 as a post-mortem biomarker of disease progression.
PB MDPI
LK https://hdl.handle.net/10612/18867
UL https://hdl.handle.net/10612/18867
NO Blanco Vázquez, C., Balseiro, A., Alonso-Hearn, M., Juste, R. A., Iglesias, N., Canive, M., & Casais, R. (2021). Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression. Animals, 11(5). https://doi.org/10.3390/ANI11051370
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 15-jun-2024