RT info:eu-repo/semantics/article
T1 Necrotic and cytolytic activity on grapevine leaves produced by Nep1-like proteins of Diplodia seriata
A1 Cobos Román, Rebeca
A1 Calvo Peña, Carla
A1 Álvarez Pérez, José Manuel
A1 Ibáñez Sánchez, Ana María
A1 Diez Galán, Alba
A1 González García, Sandra
A1 García Angulo, Penélope
A1 Acebes Arranz, José Luis
A1 Rubio Coque, Juan José
A2 Microbiologia
K1 Biología
K1 Botryosphaeriaceae
K1 NLP
K1 RT-qPCR
K1 Grapevine trunk diseases
K1 Phytotoxicity
K1 Vitis vinifera
K1 3108 Fitopatología
K1 2414 Microbiología
AB [EN] Many phytopathogenic fungi produce necrosis and ethylene inducing peptide 1 (Nep1- like proteins or NLP) that trigger leaf necrosis and the activation of defense mechanisms. These proteins have been widely studied in plant pathogens as Moniliophthora perniciosa or Botrytis cinerea between others, but little is known about their biological roles in grapevine trunk pathogens. Advances in the sequencing of genomes of several fungi involved in grapevine trunk diseases have revealed that these proteins are present in several copies in their genomes. The aim of this project was to analyze the presence of genes encoding NLP proteins in the Diplodia seriata genome and to characterize their putative role as virulence factors associated to grapevine trunk diseases. In this study, we characterized four NLPs from Diplodia seriata. All proteins showed highly similar amino acid sequences and contained the characteristic peptide motifs of NLPs. DserNEPs slightly reduced the viability of Vitis vinifera L. cell cultures. The cytolytic activity from DserNEP1 was stronger than that from DserNEP2, even at low concentrations. Purified DserNEPs also produced necrosis in leaves when they were inoculated into micropropagules of V. vinifera L. This is the first record of Nep1-like proteins from a fungus associated with grapevine trunk diseases and also from a member of the Botryosphaeriaceae family
PB Frontiers
LK https://hdl.handle.net/10612/18938
UL https://hdl.handle.net/10612/18938
NO Cobos, R., Calvo-Peña, C., Álvarez-Pérez, J. M., Ibáñez, A., Diez-Galán, A., González-García, S., García-Angulo, P., Acebes, J. L., & Coque, J. J. R. (2019). Necrotic and cytolytic activity on grapevine leaves produced by nep1-like proteins of Diplodia seriata. Frontiers in Plant Science, 10, Article e1282. https://doi.org/10.3389/FPLS.2019.01282
NO This article is part of the Research Topic Recent Advances on Grapevine-Microbe Interactions: From Signal Perception to Resistance Response
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 17, 2024