RT info:eu-repo/semantics/article
T1 Vascular wall injury and inflammation are key pathogenic mechanisms responsible for early testicular degeneration during acute besnoitiosis in bulls
A1 González Barrio, David
A1 Diezma Díaz, Carlos
A1 Tabanera, Enrique
A1 Aguado Criado, Elena
A1 Pizarro, Manuel
A1 González Huecas, Marta
A1 Ferre, Ignacio
A1 Jiménez Meléndez, Alejandro
A1 Criado, Fernando
A1 Gutiérrez Expósito, Daniel
A1 Ortega Mora, Luis Miguel
A1 Álvarez García, Gema
A2 Sanidad Animal
K1 Sanidad animal
K1 Besnoitia besnoiti
K1 Bull
K1 Acute besnoitiosis
K1 Testicular degeneration
K1 Lesions
K1 3109 Ciencias Veterinarias
AB [EN] BACKGROUND: Bovine besnoitiosis, caused by the apicomplexan parasite Besnoitia besnoiti, is a chronic and debilitating cattle disease that notably impairs fertility. Acutely infected bulls may develop respiratory signs and orchitis, and sterility has been reported in chronic infections. However, the pathogenesis of acute disease and its impact on reproductive function remain unknown. METHODS: Herein, we studied the microscopic lesions as well as parasite presence and load in the testis (pampiniform plexus, testicular parenchyma and scrotal skin) of seven bulls with an acute B. besnoiti infection. Acute infection was confirmed by serological techniques (IgM seropositive results and IgG seronegative results) and subsequent parasite detection by PCR and histological techniques. RESULTS: The most parasitized tissue was the scrotal skin. Moreover, the presence of tachyzoites, as shown by immunohistochemistry, was associated with vasculitis, and three bulls had already developed juvenile tissue cysts. In all animals, severe endothelial injury was evidenced by marked congestion, thrombosis, necrotizing vasculitis and angiogenesis, among others, in the pampiniform plexus, testicular parenchyma and scrotal skin. Vascular lesions coexisted with lesions characteristic of a chronic infection in the majority of bulls: hyperkeratosis, acanthosis and a marked diffuse fibroplasia in the dermis of the scrotum. An intense inflammatory infiltrate was also observed in the testicular parenchyma accompanied by different degrees of germline atrophy in the seminiferous tubules with the disappearance of various strata of germ cells in four bulls. CONCLUSIONS: This study confirmed that severe acute besnoitiosis leads to early sterility that might be permanent, which is supported by the severe lesions observed. Consequently, we hypothesized that testicular degeneration might be a consequence of (i) thermoregulation failure induced by vascular lesions in pampiniform plexus and scrotal skin lesions; (ii) severe vascular wall injury induced by the inflammatory response in the testis; and (iii) blood-testis barrier damage and alteration of spermatogenesis by immunoresponse
PB BMC
LK https://hdl.handle.net/10612/19123
UL https://hdl.handle.net/10612/19123
NO González Barrio, D., Diezma Díaz, C., Tabanera, E., Aguado Criado, E., Pizarro, M., González-Huecas, M., Ferre, I., Jiménez Meléndez, A., Criado, F., Gutiérrez Expósito, D., Ortega Mora, L. M., & Álvarez García, G. (2020). Vascular wall injury and inflammation are key pathogenic mechanisms responsible for early testicular degeneration during acute besnoitiosis in bulls. Parasites & vectors, 13(1), 113. https://doi.org/10.1186/S13071-020-3959-9
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 16-may-2024