RT info:eu-repo/semantics/article
T1 Phenotypic Characterization of Encephalitis and Immune Response in the Brains of Lambs Experimentally Infected with Spanish Goat Encephalitis Virus
A1 Martinez, Ileana Z.
A1 Pérez Martínez, Claudia
A1 Salinas Rodríguez, Luis Manuel
A1 Juste, Ramón A.
A1 García Marín, Juan Francisco
A1 Balseiro Morales, Ana María
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Spanish goat encephalitis virus (SGEV)
K1 Goat
K1 Lambs
K1 Cell population
K1 Immunohistochemistry
K1 3109 Ciencias Veterinarias
K1 3104.07 Ovinos
AB [EN]Spanish goat encephalitis virus (SGEV), a novel subtype of tick-borne flavivirus closelyrelated to louping ill virus, causes a neurological disease in experimentally infected goats and lambs.Here, the distribution of microglia, T and B lymphocytes, and astrocytes was determined in theencephalon and spinal cord of eight Assaf lambs subcutaneously infected with SGEV. Cells wereidentified based on immunohistochemical staining against Iba1 (microglia), CD3 (T lymphocytes),CD20 (B lymphocytes), and glial fibrillary acidic protein (astrocytes). In glial foci and perivascularcu ng areas, microglia were the most abundant cell type (45.4% of immunostained cells), followedby T lymphocytes (18.6%) and B lymphocytes (4.4%). Thalamus, hypothalamus, corpus callosum,and medulla oblongata contained the largest areas occupied by glial foci. Reactive astrogliosisoccurred to a greater extent in the lumbosacral spinal cord than in other regions of the central nervoussystem. Lesions were more frequent on the side of the animal experimentally infected with the virus.Lesions were more severe in lambs than in goats, suggesting that lambs may be more susceptibleto SGEV, which may be due to species di erences or to interindividual di erences in the immuneresponse, rather than to di erences in the relative proportions of immune cells. Larger studies thatmonitor natural or experimental infections may help clarify local immune responses to this flavivirussubtype in the central nervous system.
PB MDPI
LK https://hdl.handle.net/10612/19167
UL https://hdl.handle.net/10612/19167
NO Martínez, I. Z., Pérez‐martínez, C., Salinas, L. M., Juste, R. A., García Marín, J. F., & Balseiro, A. (2020). Phenotypic characterization of encephalitis and immune response in the brains of lambs experimentally infected with Spanish goat encephalitis virus. Animals, 10(8), Article e1373. https://doi.org/10.3390/ANI10081373
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-jun-2024