RT info:eu-repo/semantics/article
T1 Biodegradation of Pine Processionary Caterpillar Silk Is Mediated by Elastase- and Subtilisin-like Proteases
A1 Diez Galán, Alba
A1 Cobos Román, Rebeca
A1 Ibáñez Sánchez, Ana María
A1 Calvo Peña, Carla
A1 Rubio Coque, Juan José
A2 Microbiologia
K1 Biología
K1 Bioquímica
K1 Biotecnología
K1 Silk
K1 Pine processionary
K1 Bacillus
K1 Pseudomonas
K1 Biodegradation
K1 Proteases
K1 Elastase
K1 Subtilisin
K1 2403 Bioquímica
K1 3108.02 Control Biológico de Enfermedades
K1 3302.90 Ingeniería Bioquímica
K1 3308.03 Tecnología del Control de Insectos
AB [EN] Pine processionary caterpillar nests are made from raw silk. Fibroin protein is the main component of silk which, in the case of pine processionary caterpillar, has some unusual properties such as a higher resistance to chemical hydrolysis. Isolation of microorganisms naturally present in silk nests led to identification of Bacillus licheniformis and Pseudomonas aeruginosa strains that in a defined minimal medium were able to carry out extensive silk biodegradation. A LasB elastase-like protein from P. aeruginosa was shown to be involved in silk biodegradation. A recombinant form of this protein expressed in Escherichia coli and purified by affinity chromatography was able to efficiently degrade silk in an in vitro assay. However, silk biodegradation by B. licheniformis strain was mediated by a SubC subtilisin-like protease. Homologous expression of a subtilisin Carlsberg encoding gene (subC) allowed faster degradation compared to the biodegradation kinetics of a wildtype B. licheniformis strain. This work led to the identification of new enzymes involved in biodegradation of silk materials, a finding which could lead to possible applications for controlling this pest and perhaps have importance from sanitary and biotechnological points of view
PB MDPI
SN 1661-6596
LK https://hdl.handle.net/10612/19219
UL https://hdl.handle.net/10612/19219
NO Diez-Galán, A., Cobos, R., Ibañez, A., Calvo-Peña, C., & Coque, J. J. R. (2022). Biodegradation of pine processionary caterpillar silk is mediated by elastase- and subtilisin-like proteases. International Journal of Molecular Sciences, 23(23), Article e15253. https://doi.org/10.3390/ijms232315253
NO This article belongs to the Section Biochemistry
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 15-may-2024