RT info:eu-repo/semantics/article
T1 In Vitro Generation of Zebrafish PGC-Like Cells
A1 Fernández Riesco, Marta
A1 García Valcarce, David
A1 Alfonso Cendón, Javier
A1 Herráez Ortega, María Paz
A1 Robles Rodríguez, Vanesa
A2 Biologia Celular
K1 Biología
K1 Biotecnología
K1 Embryonic cell differentiation
K1 Gamete biology
K1 Primordial germ cells
K1 Zebrafish
K1 2407 Biología Celular
K1 3104.11 Reproducción
K1 2401.07 Embriología Animal
K1 2407.01 Cultivo Celular
AB [EN] The possibility of generating primordial germ cells (PGCs) in vitro from noncommitted embryonic cells represents an extremely useful tool in current research. Primordial germ cell in vitro differentiation has been successfully reported in mammals. However, contrary to fish, PGC specification in mammals is an inductive mechanism. This study is the first to date to describe a rapid method for PGC in vitro differentiation in teleosts. Primordial germ cell-like cells were characterized by several lines of evidence, including gene expression, cell complexity, size, and image analysis for the quantification of fluorescence under vasa promoter. Moreover, differentiated cells were able to colonize the genital ridge after transplantation. Differentiation treatments increased the number of PGCs in culture, causing differentiation of cells rather than inducing their proliferation. These results open up the possibility of differentiating genetically modified embryonic cells to PGC-like cells to ensure their transmission to the progeny and could be crucial for an in-depth understanding of germline differentiation in teleosts
PB Oxford University Press
SN 0006-3363
LK https://hdl.handle.net/10612/19309
UL https://hdl.handle.net/10612/19309
NO Riesco, M. F., Valcarce, D. G., Alfonso, J., Herráez, M. P., & Robles, V. (2014). In vitro generation of zebrafish PGC-like cells. Biology of Reproduction, 91(5), Article e114 https://doi.org/10.1095/BIOLREPROD.114.121491
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 22-may-2024