RT info:eu-repo/semantics/article
T1 Field-Deployed Spectroscopy from 350 to 2500 nm: A Promising Technique for Early Identification of Powdery Mildew Disease (Erysiphe necator) in Vineyards
A1 Vélez, Sergio
A1 Barajas, Enrique
A1 Rubio, José Antonio
A1 Pereira Obaya, Dimas
A1 Rodríguez Pérez, José Ramón
A2 Ingeniería CartograficaGeodesica y Fotogrametria
K1 Ingeniería agrícola
K1 Erysiphe aka Uncinula necator
K1 Early detection
K1 Vegetation health monitoring
K1 Tempranillo
K1 Plant stress
K1 Non-invasive
K1 Spectral signature
K1 Pathogen detection
K1 Precision agriculture
K1 Proximal sensing
K1 3103
AB [EN] This study explores spectroscopy in the 350 to 2500 nm range for detecting powdery mildew (Erysiphe necator) in grapevine leaves, crucial for precision agriculture and sustainable vineyard management. In a controlled experimental vineyard setting, the spectral reflectance on leaves with varying infestation levels was measured using a FieldSpec 4 spectroradiometer during July and September. A detailed assessment was conducted following the guidelines recommended by the European and Mediterranean Plant Protection Organization (EPPO) to quantify the level of infestation; categorising leaves into five distinct grades based on the percentage of leaf surface area affected. Subsequently, spectral data were collected using a contact probe with a tungsten halogen bulb connected to the spectroradiometer, taking three measurements across different areas of each leaf. Partial Least Squares Regression (PLSR) analysis yielded coefficients of determination R2 = 0.74 and 0.71, and Root Mean Square Errors (RMSEs) of 12.1% and 12.9% for calibration and validation datasets, indicating high accuracy for early disease detection. Significant spectral differences were noted between healthy and infected leaves, especially around 450 nm and 700 nm for visible light, and 1050 nm, 1425 nm, 1650 nm, and 2250 nm for the near-infrared spectrum, likely due to tissue damage, chlorophyll degradation and water loss. Finally, the Powdery Mildew Vegetation Index (PMVI) was introduced, calculated as PMVI = (R755 − R675)/(R755 + R675), where R755 and R675 are the reflectances at 755 nm (NIR) and 675 nm (red), effectively estimating disease severity (R2 = 0.7). The study demonstrates that spectroscopy, combined with PMVI, provides a reliable, non-invasive method for managing powdery mildew and promoting healthier vineyards through precision agriculture practices.
PB MDPI
SN 2073-4395
LK https://hdl.handle.net/10612/19332
UL https://hdl.handle.net/10612/19332
NO Vélez, S.; Barajas, E.; Rubio, J.A.; Pereira-Obaya, D.; Rodríguez-Pérez, J.R. Field-Deployed Spectroscopy from 350 to 2500 nm: A Promising Technique for Early Identification of Powdery Mildew Disease (Erysiphe necator) in Vineyards. Agronomy 2024, 14, 634. https://doi.org/10.3390/agronomy14030634
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024