RT info:eu-repo/semantics/article
T1 Early Salmonella Typhimurium infection in pigs disrupts Microbiome composition and functionality principally at the ileum mucosa
A1 Argüello Rodríguez, Héctor
A1 Estellé, Jordi
A1 Zaldívar López, Sara
A1 Jiménez Marín, Ángeles
A1 Carvajal Urueña, Ana María
A1 López Bascón, María Asunción
A1 Crispie, Fiona
A1 O´Sullivan, Orla
A1 Cotter, Paul D.
A1 Priego Capote, Feliciano
A1 Morera, Luis
A1 Garrido, Juan J.
A2 Sanidad Animal
K1 Sanidad animal
K1 Early Salmonella Typhimurium infection
K1 Pigs
K1 3109 Ciencias Veterinarias
AB [EN] Salmonella is a major foodborne pathogen which successfully infects animal species for human consumption such as swine. The pathogen has a battery of virulence factors which it uses to colonise and persist within the host. The host microbiota may play a role in resistance to, and may also be indirectly responsible from some of the consequences of, Salmonella infection. To investigate this, we used 16S rRNA metagenomic sequencing to determine the changes in the gut microbiota of pigs in response to infection by Salmonella Typhimurium at three locations: ileum mucosa, ileum content and faeces. Early infection (2 days post-infection) impacted on the microbiome diversity at the mucosa, reflected in a decrease in representatives of the generally regarded as desirable genera (i.e., Bifidobacterium and Lactobacillus). Severe damage in the epithelium of the ileum mucosa correlated with an increase in synergistic (with respect to Salmonella infection; Akkermansia) or opportunistically pathogenic bacteria (Citrobacter) and a depletion in anaerobic bacteria (Clostridium spp., Ruminococcus, or Dialliser). Predictive functional analysis, together with metabolomic analysis revealed changes in glucose and lipid metabolism in infected pigs. The observed changes in commensal healthy microbiota, including the growth of synergistic or potentially pathogenic bacteria and depletion of beneficial or competing bacteria, could contribute to the pathogen's ability to colonize the gut successfully. The findings from this study could be used to form the basis for further research aimed at creating intervention strategies to mitigate the effects of Salmonella infection
PB Nature Research
LK https://hdl.handle.net/10612/19393
UL https://hdl.handle.net/10612/19393
NO Argüello, H., Estellé, J., Zaldívar-López, S., Jiménez-Marín, Á., Carvajal, A., López-Bascón, M. A., Crispie, F., O’Sullivan, O., Cotter, P. D., Priego-Capote, F., Morera, L., & Garrido, J. J. (2018). Early Salmonella Typhimurium infection in pigs disrupts Microbiome composition and functionality principally at the ileum mucosa. Scientific Reports, 8(1). https://doi.org/10.1038/S41598-018-26083-3
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024