RT info:eu-repo/semantics/article
T1 Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
A1 Balseiro Morales, Ana María
A1 Altuzarra, Raúl
A1 Vidal, Enric
A1 Moll, Xavier
A1 Espada, Yvonne
A1 Sevilla, Iker A.
A1 Domingo, Mariano
A1 Garrido, Joseba M.
A1 Juste, Ramón A.
A1 Prieto, Miguel
A1 Pérez de Val, Bernat
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 BCG vaccine
K1 Mycobacterium bovis
K1 Vacunas (Veterinaria)
K1 Ganado ovino
K1 3109 Ciencias Veterinarias
K1 3104.07 Ovinos
K1 2412.10 Vacunas
AB [EN]Background/Aims: Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study were to develop an experimental TB infection model in sheep with a Mycobacterium caprae field strain isolated from a tuberculous diseased ewe, and to use this to evaluate the safety and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. Methods: Eighteen 2 month-old lambs were experimentally challenged with M. caprae by the endotracheal route (1.5 × 103 CFU). They were separated per treatment group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n = 6). Clinical, immunological, pathological and bacteriological parameters of infection were measured. Results: All lambs were successfully infected and developed gross TB lesions in the respiratory system. The BCG vaccine conferred considerable protection against experimental TB in lambs, as measured by a reduction of the gross lesion volumes and bacterial load. However, HIMB vaccinated animals did not show protection. Conclusions: This study proposes a reliable new experimental model for a better understanding of tuberculosis in sheep. BCG vaccination offers an effective prospect for controlling the disease. Moreover alternative doses and/or routes of administration should be considered to evaluate the efficacy of the HIMB vaccine candidate.
PB Public Library of Science
LK https://hdl.handle.net/10612/19718
UL https://hdl.handle.net/10612/19718
NO Balseiro, A., Altuzarra, R., Vidal, E., Moll, X., Espada, Y., Sevilla, I. A., Domingo, M., Garrido, J. M., Juste, R. A., Prieto, M., & Pérez de Val, B. (2017). Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep. PLoS ONE, 12(7), Article e0180546. https://doi.org/10.1371/JOURNAL.PONE.0180546
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 29-jun-2024