RT info:eu-repo/semantics/article
T1 New insights about functional and cross-reactive properties of antibodies generated against recombinant TbpBs of Haemophilus parasuis
A1 Barasuol, Bibiana Martins
A1 Guizzo, João Antônio
A1 Fegan, Jamie Elisabeth
A1 Martínez Martínez, Sonia
A1 Rodríguez Ferri, Elías Fernando
A1 Gutiérrez Martín, César Bernardo
A1 Kreutz, Luiz Carlos
A1 Schryvers, Anthony Bernard
A1 Frandoloso, Rafael
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Vaccines
K1 Glässer Disease
K1 Haemophilus parasuis
K1 Pig
K1 N-lobe
K1 C-lobe
K1 Protein B
K1 TbpB
K1 Transferrin
K1 2412.10 Vacunas
K1 3109 Ciencias Veterinarias
K1 32 Ciencias Médicas
AB [EN] Vaccines have become fundamental in the control and elimination of Glässer Disease, a systemic disease of pigs caused by Haemophilus parasuis. The classic vaccines available for prevention of this infection were developed without a robust knowledge about host immunological mechanisms. In this study, we demonstrated the presence of cross-reactive epitopes on both the N-lobe and C-lobe of variants of transferrin binding protein B (TbpBs) expressed on the surface of 6 virulent serovars of H. parasuis. Antibodies against TbpB-derived antigens were capable of increasing the phagocytic capacity of neutrophils and were also capable of blocking porcine transferrin from binding to TbpB. Surprisingly, none of the pig or mice antisera from animals immunized with TbpB-derived antigens mixed with Montanide IMS 2215 VG PR adjuvant were able to activate the classical complement pathway (CCP). In contrast, antisera from mice immunized with TbpB-derived antigens adjuvanted with Freund's adjuvants or Montanide Gel 01 were able to activate the CCP and kill H. parasuis. Our results demonstrate that the type of adjuvant can modulate the functional response induced by TbpB-derived antigens. Based on these results, we propose that a properly formulated TbpB-based vaccine may elicit a functional protective antibody response with broad cross-reactivity against heterologous strains of H. parasuis.
PB Nature Research
LK https://hdl.handle.net/10612/19723
UL https://hdl.handle.net/10612/19723
NO Barasuol, B. M., Guizzo, J. A., Fegan, J. E., Martínez-Martínez, S., Rodríguez-Ferri, E. F., Gutiérrez-Martín, C. B., Kreutz, L. C., Schryvers, A. B., & Frandoloso, R. (2017). New insights about functional and cross-reactive properties of antibodies generated against recombinant TbpBs of Haemophilus parasuis. Scientific Reports, 7(1), Article e10377. https://doi.org/10.1038/S41598-017-10627-0
NO Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 17-may-2024