RT info:eu-repo/semantics/article
T1 Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates
A1 Frey, Caroline F.
A1 Regidor Cerrillo, Javier
A1 Marreros, Nelson
A1 García Lunar, Paula
A1 Gutiérrez Expósito, Daniel
A1 Schares, Gereon
A1 Dubey, Jitender P.
A1 Gentile, Arcangelo
A1 Jacquiet, Philippe
A1 Shkap, Varda
A1 Cortes, Helder
A1 Ortega Mora, Luis Miguel
A1 Álvarez García, Gemma
A2 Sanidad Animal
K1 Veterinaria
K1 Besnoitia besnoiti
K1 Besnoitia tarandi
K1 Lytic cycle
K1 In vitro model
K1 Isolates
K1 Invasion
K1 Proliferation
K1 3109 Ciencias Veterinarias
K1 2401.12 Parasitología Animal
AB [EN]Background: Bovine besnoitiosis, caused by the protozoan Besnoitia besnoiti, reduces productivity and fertility of affected herds. Besnoitiosis continues to expand in Europe and no effective control tools are currently available. Experimental models are urgently needed. Herein, we describe for the first time the kinetics of standardised in vitro models for the B. besnoiti lytic cycle. This will aid to study the pathogenesis of the disease, in the screening for vaccine targets and drugs potentially useful for the treatment of besnoitiosis. Methods: We compared invasion and proliferation of one B. tarandi (from Finland) and seven B. besnoiti isolates (Bb-Spain1, Bb-Spain2, Bb-Israel, Bb-Evora03, Bb-Ger1, Bb-France, Bb-Italy2) in MARC-145 cell culture. Host cell invasion was studied at 4, 6, 8 and 24 h post infection (hpi), and proliferation characteristics were compared at 24, 48, 72, 96, 120, and 144 hpi. Results: In Besnoitia spp., the key parameters that determine the sequential adhesion-invasion, proliferation and egress steps are clearly distinct from those in the related apicomplexans Toxoplasma gondii and Neospora caninum. Besnoitia spp. host cell invasion is a rather slow process, since only 50 % of parasites were found intracellular after 3-6 h of exposure to host cells, and invasion still took place after 24 h. Invasion efficacy was significantly higher for Bb-France, Bb-Evora03 and Bb-Israel. In addition, the time span for endodyogeny to take place was as long as 18-35 h. Bb-Israel and B. tarandi isolates were most prolific, as determined by the tachyzoite yield at 72 hpi. The total tachyzoite yield could not be predicted neither by invasion-related parameters (velocity and half time invasion) nor by proliferation parameters (lag phase and doubling time (dT)). The lytic cycle of Besnoitia was asynchronous as evidenced by the presence of three different plaque-forming tachyzoite categories (lysis plaques, large and small parasitophorous vacuoles). Conclusions: This study provides first insights into the lytic cycle of B. besnoiti isolates and a standardised in vitro model that allows screening of drug candidates for the treatment of besnoitiosis.
PB BMC
LK https://hdl.handle.net/10612/19864
UL https://hdl.handle.net/10612/19864
NO Frey, C. F., Regidor-Cerrillo, J., Marreros, N., García-Lunar, P., Gutiérrez-Expósito, D., Schares, G., Dubey, J. P., Gentile, A., Jacquiet, P., Shkap, V., Cortes, H., Ortega-Mora, L. M., & Álvarez-García, G. (2016). Besnoitia besnoiti lytic cycle in vitro and differences in invasion and intracellular proliferation among isolates. Parasites and Vectors, 9, Article e115. https://doi.org/10.1186/S13071-016-1405-9
NO © 2016 Frey et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 29-jun-2024