RT info:eu-repo/semantics/article
T1 The European antibody network's practical guide to finding and validating suitable antibodies for research
A1 Roncador, Giovanna
A1 Engel, Pablo
A1 Maestre, Lorena
A1 Anderson, Amanda P.
A1 Cordell, Jacqueline L.
A1 Cragg, Mark S.
A1 Šerbec, Vladka Č.
A1 Jones, Margaret
A1 Lisnic, Vanda J.
A1 Kremer, Leonor
A1 Li, Demin
A1 Koch-Nolte, Friedrich
A1 Pascual, Núria
A1 Rodríguez Barbosa, José Ignacio
A1 Torensma, Ruurd
A1 Turley, Helen
A1 Pulford, Karen
A1 Banham, Alison H.
A2 Sanidad Animal
K1 Inmunología
K1 Veterinaria
K1 BLAST searches
K1 CiteAb
K1 EuroMAbNet
K1 Flow cytometry
K1 Gene silencing
K1 HLDA workshops
K1 Immunohistochemistry
K1 Monoclonal antibodies
K1 Validation studies
K1 Western blotting
K1 3109 Ciencias Veterinarias
K1 3109.03 Inmunología
K1 2412 Inmunología
AB [EN]Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.
PB Taylor and Francis Group
SN 1942-0862
LK https://hdl.handle.net/10612/19897
UL https://hdl.handle.net/10612/19897
NO Roncador, G., Engel, P., Maestre, L., Anderson, A. P., Cordell, J. L., Cragg, M. S., Šerbec, V. Č., Jones, M., Lisnic, V. J., Kremer, L., Li, D., Koch-Nolte, F., Pascual, N., Rodríguez Barbosa, J.-I., Torensma, R., Turley, H., Pulford, K., & Banham, A. H. (2016). The European antibody network’s practical guide to finding and validating suitable antibodies for research. mAbs, 8(1), 27-36. https://doi.org/10.1080/19420862.2015.1100787
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 22-may-2024