RT info:eu-repo/semantics/article T1 Evaluation of the application for new alternative biodiesel production process for rendered fat including Category 1 animal by‐products (BDI‐RepCat® process, AT) A1 Koutsoumanis, Konstantinos A1 Allende, Ana A1 Bolton, Declan A1 Bover‐Cid, Sara A1 Chemaly, Marianne A1 Davies, Robert A1 De Cesare, Alessandra A1 Herman, Lieve A1 Hilbert, Friederike A1 Lindqvist, Roland A1 Nauta, Maarten A1 Peixe, Luisa A1 Ru, Giuseppe A1 Simmons, Marion A1 Skandamis, Panagiotis A1 Suffredini, Elisabetta A1 Fernández Escámez, Pablo A1 Spiropoulos, John A1 Iulietto, Maria Francesca A1 Ortiz Peláez, Ángel A1 Álvarez Ordóñez, Avelino A2 Tecnologia de los Alimentos K1 Bioquímica K1 Animal by-product K1 ABP K1 Biodiesel K1 Prion K1 TSE K1 Methanol K1 Tallow K1 Category 1 K1 2302 Bioquímica AB [EN] A new alternative method for the production of biodiesel from rendered fat, including animal by-product (ABP) Category 1 tallow, was evaluated. The method consists of a conversion phase, based on esterification and transesterification in a single step (at temperature ≥ 200°C, pressure ≥ 70 bar with a retention time ≥ 15 min), using MgO as a catalyst and in the presence of methanol (10–15%), followed by vacuum distillation (at ≥ 150°C, ≤ 10 mbar) of the end-product, biodiesel and the co-product, glycerine. Prions (PrPSc), which are abnormal isoforms of the prion protein, were considered by the applicant to be the most resistant hazard. In accordance with previous EFSA Opinions and current expert evaluation, a reduction in prion infectivity, or detectable PrPSc, of at least 6 log10 should be achieved for the process to be considered equivalent to the processing method laid down in the Regulation (EU) No 142/2011. Published data from an experimental replication of the conversion step of the biodiesel production process under consideration were provided, which showed an at least 6 log10 reduction in detectable PrPSc, by Western blot, in tallow that had been spiked with murine and human prion strains. In addition, it was demonstrated that the presence of methanol does not affect the recovery or detection of PrPSc from a biodiesel substrate. Based on scientific literature, the vacuum distillation step has been shown to be capable of achieving an additional 3 log10 reduction in PrPSc. Therefore, the proposed alternative method is considered to be at least equivalent to the processing method laid down in the legislation for the production of biodiesel from raw materials including Category 1 ABP PB Wiley SN 1831-4732 LK https://hdl.handle.net/10612/20637 UL https://hdl.handle.net/10612/20637 NO Koutsoumanis, K., Allende, A., Bolton, D., Bover-Cid, S., Chemaly, M., Davies, R., De Cesare, A., Herman, L., Hilbert, F., Lindqvist, R., Nauta, M., Peixe, L., Ru, G., Simmons, M., Skandamis, P., Suffredini, E., Fernández Escámez, P., Spiropoulos, J., Iulietto, M. F., et al. (2021). Evaluation of the application for new alternative biodiesel production process for rendered fat including Category 1 animal by-products (BDI-RepCat® process, AT). EFSA Journal, 19(4). https://doi.org/10.2903/J.EFSA.2021.6511 DS BULERIA. Repositorio Institucional de la Universidad de León RD 19-jun-2024