RT info:eu-repo/semantics/article
T1 A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan
A1 Leeuwea, Tim M. van
A1 Wattjesb, Jasper
A1 Niehues, Anna
A1 Forn Cuní, Gabriel
A1 Geoffriond, Nicholas
A1 Mélida Martínez, Hugo
A1 Arentshorsta, Mark
A1 Molina, Antonio
A1 Tsangd, Adrian
A1 Meijer, Annemarie H.
A1 Moerschbacher, Bruno M.
A1 Punt, Peter J.
A1 Ram, Arthur F.J.
A2 Fisiologia Vegetal
K1 Ingeniería agrícola
K1 Chitin
K1 Crh transglycosylases
K1 α-glucan
K1 Cell wall integrity
K1 Aspergillus niger
K1 3102 Ingeniería Agrícola
K1 2417.19 Fisiología Vegetal
AB [EN] Chitin is an important fungal cell wall component that is cross-linked to β-glucan for structural integrity.Acquisition of chitin to glucan cross-links has previously been shown to be performed by transglycosylationenzymes in Saccharomyces cerevisiae, called Congo Red hypersensitive (Crh) enzymes. Here, we characterized theimpact of deleting all seven members of the crh gene family (crhA-G) in Aspergillus niger on cell wall integrity, cellwall composition and genome-wide gene expression. In this study, we show that the seven-fold crh knockoutstrain shows slightly compact growth on plates, but no increased sensitivity to cell wall perturbing compounds.Additionally, we found that the cell wall composition of this knockout strain was virtually identical to that of thewild type. In congruence with these data, genome-wide expression analysis revealed very limited changes ingene expression and no signs of activation of the cell wall integrity response pathway. However, deleting theentire crh gene family in cell wall mutants that are deficient in either galactofuranose or α-glucan, mainly α-1,3-glucan, resulted in a synthetic growth defect and an increased sensitivity towards Congo Red compared to theparental strains, respectively. Altogether, these results indicate that loss of the crh gene family in A. niger doesnot trigger the cell wall integrity response, but does play an important role in ensuring cell wall integrity inmutant strains with reduced galactofuranose or α-glucan.
PB Elsevier
SN 2468-2330
LK https://hdl.handle.net/10612/20722
UL https://hdl.handle.net/10612/20722
NO van Leeuwe, T. M., Wattjes, J., Niehues, A., Forn-Cuní, G., Geoffrion, N., Mélida, H., Arentshorst, M., Molina, A., Tsang, A., Meijer, A. H., Moerschbacher, B. M., Punt, P. J., & Ram, A. F. J. (2020). A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan. Cell Surface, 6. https://doi.org/10.1016/J.TCSW.2020.100039
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-jun-2024