RT info:eu-repo/semantics/article
T1 Antibacterial Action Mechanisms of Honey: Physiological Effects of Avocado, Chestnut, and Polyfloral Honey upon Staphylococcus aureus and Escherichia coli
A1 Combarros Fuertes, Patricia
A1 Estevinho, Leticia M.
A1 Teixeira Santos, Rita
A1 Rodrigues, Acácio G.
A1 Pina Vaz, Cidália
A1 Fresno Baro, José María
A1 Tornadijo Rodríguez, María Eugenia
A2 Tecnologia de los Alimentos
K1 Tecnología de los alimentos
K1 Antibacterial mechanisms
K1 Flow cytometry
K1 Avocado honey
K1 Chestnut honey
K1 Polyfloral honey
K1 Staphylococcus aureus
K1 Escherichia coli
K1 3309 Tecnología de Los Alimentos
AB [EN] Numerous studies have explored the antibacterial properties of different types of honey from all around the world. However, the data available describing how honey acts against bacteria are few. The aim of this study was to apply a flow cytometry (FC) protocol to examine and characterize the primary effects of three varieties of honey (avocado, chestnut and polyfloral) upon physiological status of Staphylococcus aureus and Escherichia coli cells to reveal their antibacterial action mechanisms. The effects of honey samples on membrane potential, membrane integrity, and metabolic activity were assessed using different fluorochromes, in a 180 min time course assay. Time-kill experiments were also carried out under similar conditions. Exposure of S. aureus and E. coli to the distinct honey samples resulted in physiological changes related to membrane polarization and membrane integrity. Moreover, honey induced a remarkable metabolic disruption as primary physiological effect upon S. aureus. The different honey samples induced quite similar effects on both bacteria. However, the depth of bacteria response throughout the treatment varied depending on the concentration tested and among honey varieties, probably due to compositional differences in the honey
PB MDPI
LK https://hdl.handle.net/10612/20792
UL https://hdl.handle.net/10612/20792
NO Combarros Fuertes, P., Estevinho, L. M., Teixeira Santos, R., Rodrigues, A. G., Pina Vaz, C., Fresno, J. M., & Eugenia Tornadijo. (2020). Antibacterial action mechanisms of honey: Physiological Effects of Avocado, Chestnut, and Polyfloral Honey upon Staphylococcus aureus and Escherichia coli. Molecules, 25(5). https://doi.org/10.3390/MOLECULES25051252
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 18-jun-2024