RT info:eu-repo/semantics/article
T1 Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β‐subunit of the heterotrimeric G‐protein
A1 Escudero, Viviana
A1 Jordá, Lucía
A1 Sopeña Torres, Sara
A1 Mélida Martínez, Hugo
A1 Miedes, Eva
A1 Muñoz Barrios, Antonio
A1 Swami, Sanjay
A1 Alexander, Danny
A1 McKee, Lauren S.
A1 Sánchez Vallet, Andrea
A1 Bulone, Vincent
A1 Jones, Alan M.
A2 Fisiologia Vegetal
K1 Ingeniería agrícola
K1 Heterotrimeric G-protein
K1 AGB1
K1 Agb1-2
K1 Plant Cell Wall
K1 Xylan
K1 Necrotrophic Fungi
K1 Immunity
K1 Pathogen-associated Molecular Pattern
K1 Plectosphaerella Cucumerina
K1 Arabidopsis Thaliana
K1 3102 Ingeniería Agrícola
K1 2417.19 Fisiología Vegetal
AB [EN] Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggeredimmunity (PTI) and disease resistance responses to different types of pathogens. It also plays a role in plantcell wall integrity as mutants impaired in the Gb-(agb1-2)orGc-subunits have an altered wall compositioncompared with wild-type plants. Here we performed a mutant screen to identify suppressors ofagb1-2(sgb) that restore susceptibility to pathogens to wild-type levels. Out of the foursgbmutants (sgb10–sgb13)identified,sgb11is a new mutant allele ofESKIMO1(ESK1), which encodes a plant-specific polysaccharideO-acetyltransferase involved in xylan acetylation. Null alleles (sgb11/esk1-7)ofESK1restore to wild-typelevels the enhanced susceptibility ofagb1-2to the necrotrophic fungusPlectosphaerella cucumerina BMM(PcBMM), but not to the bacteriumPseudomonas syringaepv.tomatoDC3000 or to the oomyceteHyaloper-onospora arabidopsidis. The enhanced resistance toPcBMMof theagb1-2 esk1-7double mutant was notthe result of the re-activation of deficient PTI responses inagb1-2. Alteration of cell wall xylan acetylationcaused byESK1impairment was accompanied by an enhanced accumulation of abscisic acid, the constitu-tive expression of genes encoding antibiotic peptides and enzymes involved in the biosynthesis of trypto-phan-derived metabolites, and the accumulation of disease resistance-related secondary metabolites anddifferent osmolites. Theseesk1-mediated responses counterbalance the defective PTI andPcBMMsuscepti-bility ofagb1-2plants, and explain the enhanced drought resistance ofesk1plants. These results suggestthat a deficient PTI-mediated resistance is partially compensated by the activation of specific cell-wall-trig-gered immune responses.
PB Wiley-Blackwell
SN 0960-7412
LK https://hdl.handle.net/10612/20795
UL https://hdl.handle.net/10612/20795
NO Escudero, V., Jordá, L., Sopeña-Torres, S., Mélida, H., Miedes, E., Muñoz-Barrios, A., Swami, S., Alexander, D., McKee, L. S., Sánchez-Vallet, A., Bulone, V., Jones, A. M., & Molina, A. (2017). Alteration of cell wall xylan acetylation triggers defense responses that counterbalance the immune deficiencies of plants impaired in the β-subunit of the heterotrimeric G-protein. Plant Journal, 92(3), 386-399. https://doi.org/10.1111/TPJ.13660
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 12, 2024