RT info:eu-repo/semantics/article
T1 Development of a qPCR Strategy to Select Bean Genes Involved in Plant Defense Response and Regulated by the Trichoderma velutinum – Rhizoctonia solani Interaction
A1 Mayo Prieto, Sara
A1 Cominelli, Eleonora
A1 Sparvoli, Francesca
A1 González López, Óscar
A1 Rodríguez González, Álvaro
A1 Gutiérrez Martínez, Santiago
A1 Casquero Luelmo, Pedro Antonio
A2 Ingenieria Agroforestal
K1 Ingeniería agrícola
K1 Ingeniería forestal
K1 Biotic Stress
K1 Systemic Acquired Resistance
K1 Induced Systemic Resistance
K1 Hypersensitive Response
K1 Defense Genes
K1 Biocontrol Agent
K1 Phaseolus Vulgaris
AB [EN] Bean production is affected by a wide diversity of fungal pathogens, among themRhizoctonia solani is one of the most important. A strategy to control bean infectiousdiseases, mainly those caused by fungi, is based on the use of biocontrol agents(BCAs) that can reduce the negative effects of plant pathogens and also can promotepositive responses in the plant. Trichoderma is a fungal genus that is able to inducethe expression of genes involved in plant defense response and also to promote plantgrowth, root development and nutrient uptake. In this article, a strategy that combinesin silico analysis and real time PCR to detect additional bean defense-related genes,regulated by the presence of Trichoderma velutinum and/or R. solani has been applied.Based in this strategy, from the 48 bean genes initially analyzed, 14 were selected, andonly WRKY33, CH5b and hGS showed an up-regulatory response in the presence ofT. velutinum. The other genes were or not affected (OSM34) or down-regulated by thepresence of this fungus. R. solani infection resulted in a down-regulation of most ofthe genes analyzed, except PR1, OSM34 and CNGC2 that were not affected, and thepresence of both, T. velutinum and R. solani, up-regulates hGS and down-regulatesall the other genes analyzed, except CH5b which was not significantly affected. Asconclusion, the strategy described in the present work has been shown to be effectiveto detect genes involved in plant defense, which respond to the presence of a BCA orto a pathogen and also to the presence of both. The selected genes show significanthomology with previously described plant defense genes and they are expressed inbean leaves of plants treated with T. velutinum and/or infected with R. solani.
PB Frontiers Media
LK https://hdl.handle.net/10612/20914
UL https://hdl.handle.net/10612/20914
NO Mayo Prieto, S.; Cominelli, E.; Sparvoli, F.; González López, Ó.; Rodríguez González, Á.; Gutiérrez Martínez, S.; Casquero Luelmo, P. A. (2016). Development of a qPCR Strategy to Select Bean Genes Involved in Plant Defense Response and Regulated by the Trichoderma velutinum – Rhizoctonia solani Interaction. Frontiers in Plant Science, 7, https://doi.org/10.3389/FPLS.2016.01109
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-jun-2024