RT info:eu-repo/semantics/article
T1 An improved micropropagation protocol for stone pine (Pinus pinea L.)
A1 Alonso, Pablo
A1 Moncaleán, Paloma
A1 Fernández, Belén
A1 Rodríguez, Ana
A1 Centeno Martín, María Luz
A1 Ordás, Ricardo J.
A2 Fisiologia Vegetal
K1 Ingeniería agrícola
K1 Conifers
K1 Micropropagation
K1 Organogenesis
K1 Rooting
K1 Tissue Culture
K1 2417.19 Fisiología Vegetal
AB [EN] A protocol for micropropagation from isolated cotyledons from Pinus pinea L. has been developed. Major improvements are the increasein rooting rates and the description for the first time of a successful continuous multiplication procedure. For bud induction, isolated cotyledons werecultured on 1/2 LP with 44.4 μM BA during 4 days. Shoot development was obtained by transfer to 1/2 LP hormone-free medium with activated charcoal.Since an additional 20 weeks were required for shoot elongation and rooting, at least 70 plantlets can be expected per seed after 29 weeks based on a70% success rate. Besides, shoots can be micropropagated successively by subculturing on 1/2 LPC.
PB Springer Nature
SN 1286-4560
LK https://hdl.handle.net/10612/21010
UL https://hdl.handle.net/10612/21010
NO Alonso, P., Moncaleán, P., Fernández, B., Rodríguez, A., Centeno, M. L., & Ordás, R. J. (2006). An improved micropropagation protocol for stone pine (Pinus pinea L.). Annals of Forest Science, 63(8), 879-885. https://doi.org/10.1051/FOREST:2006071
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-jun-2024