RT info:eu-repo/semantics/article
T1 Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis
A1 García Ulloa, Alba
A1 Sanjurjo, Laura
A1 Cimini, Sara
A1 Encina García, Antonio Esteban
A1 Martínez Rubio, Romina
A1 Bouza, Rebeca
A1 Barral, Luis
A1 Estévez Pérez, Graciela
A1 Novo Uzal
A1 Gara de, Laura
A1 Pomar, Federico
A2 Fisiologia Vegetal
K1 Ingeniería agrícola
K1 Class Iii Peroxidases
K1 Lignins
K1 Syringyl
K1 Secondary Cell Wall
K1 Redox Homeostasis
K1 Rna-seq
K1 2417.19 Fisiología Vegetal
AB [EN] Class III plant peroxidases (Prxs) are involved in the oxidative polymerization of lignins.Zinnia elegans Jacq. Basic peroxidase (ZePrx) has been previously characterized ascapable of catalyzing this reaction in vitro and the role in lignin biosynthesis of severalof its Arabidopsis thaliana homologous has been previously confirmed. In the presentwork, ZePrx was overexpressed in Nicotiana tabacum to further characterize its functionin planta with particular attention to its involvement in lignin biosynthesis. Since Prxsare known to alter ROS levels by using them as electron acceptor or producing themin their catalytic activity, the impact of this overexpression in redox homeostasis wasstudied by analyzing the metabolites and enzymes of the ascorbate-glutathione cycle.In relation to the modification induced by ZePrx overexpression in lignin compositionand cellular metabolism, the carbohydrate composition of the cell wall as well as overallgene expression through RNA-Seq were analyzed. The obtained results indicate thatthe overexpression of ZePrx caused an increase in syringyl lignin in cell wall stems,suggesting that ZePrx is relevant for the oxidation of sinapyl alcohol during ligninbiosynthesis, coherently with its S-peroxidase nature. The increase in the glucosecontent of the cell wall and the reduction of the expression of several genes involved insecondary cell wall biosynthesis suggests the occurrence of a possible compensatoryresponse to maintain cell wall properties. The perturbation of cellular redox homeostasisoccurring as a consequence of ZePrx overexpression was kept under control by anincrease in APX activity and a reduction in ascorbate redox state. In conclusion, ourresults confirm the role of ZePrx in lignin biosynthesis and highlight that its activity alterscellular pathways putatively aimed at maintaining redox homeostasis.
PB Frontiers Media
LK https://hdl.handle.net/10612/21195
UL https://hdl.handle.net/10612/21195
NO García-Ulloa, A., Sanjurjo, L., Cimini, S., Encina, A., Martínez-Rubio, R., Bouza, R., Barral, L., Estévez-Pérez, G., Novo-Uzal, E., De Gara, L., & Pomar, F. (2020). Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis. Frontiers in Plant Science, 11. https://doi.org/10.3389/FPLS.2020.00900
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 29-jun-2024