RT info:eu-repo/semantics/article T1 Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis A1 García Ulloa, Alba A1 Sanjurjo, Laura A1 Cimini, Sara A1 Encina García, Antonio Esteban A1 Martínez Rubio, Romina A1 Bouza, Rebeca A1 Barral, Luis A1 Estévez Pérez, Graciela A1 Novo Uzal A1 Gara de, Laura A1 Pomar, Federico A2 Fisiologia Vegetal K1 Ingeniería agrícola K1 Class Iii Peroxidases K1 Lignins K1 Syringyl K1 Secondary Cell Wall K1 Redox Homeostasis K1 Rna-seq K1 2417.19 Fisiología Vegetal AB [EN] Class III plant peroxidases (Prxs) are involved in the oxidative polymerization of lignins.Zinnia elegans Jacq. Basic peroxidase (ZePrx) has been previously characterized ascapable of catalyzing this reaction in vitro and the role in lignin biosynthesis of severalof its Arabidopsis thaliana homologous has been previously confirmed. In the presentwork, ZePrx was overexpressed in Nicotiana tabacum to further characterize its functionin planta with particular attention to its involvement in lignin biosynthesis. Since Prxsare known to alter ROS levels by using them as electron acceptor or producing themin their catalytic activity, the impact of this overexpression in redox homeostasis wasstudied by analyzing the metabolites and enzymes of the ascorbate-glutathione cycle.In relation to the modification induced by ZePrx overexpression in lignin compositionand cellular metabolism, the carbohydrate composition of the cell wall as well as overallgene expression through RNA-Seq were analyzed. The obtained results indicate thatthe overexpression of ZePrx caused an increase in syringyl lignin in cell wall stems,suggesting that ZePrx is relevant for the oxidation of sinapyl alcohol during ligninbiosynthesis, coherently with its S-peroxidase nature. The increase in the glucosecontent of the cell wall and the reduction of the expression of several genes involved insecondary cell wall biosynthesis suggests the occurrence of a possible compensatoryresponse to maintain cell wall properties. The perturbation of cellular redox homeostasisoccurring as a consequence of ZePrx overexpression was kept under control by anincrease in APX activity and a reduction in ascorbate redox state. In conclusion, ourresults confirm the role of ZePrx in lignin biosynthesis and highlight that its activity alterscellular pathways putatively aimed at maintaining redox homeostasis. PB Frontiers Media LK https://hdl.handle.net/10612/21195 UL https://hdl.handle.net/10612/21195 NO García-Ulloa, A., Sanjurjo, L., Cimini, S., Encina, A., Martínez-Rubio, R., Bouza, R., Barral, L., Estévez-Pérez, G., Novo-Uzal, E., De Gara, L., & Pomar, F. (2020). Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis. Frontiers in Plant Science, 11. https://doi.org/10.3389/FPLS.2020.00900 DS BULERIA. Repositorio Institucional de la Universidad de León RD 26-jun-2024