RT info:eu-repo/semantics/article T1 Influence of Fungicide Application and Vine Age on Trichoderma Diversity as Source of Biological Control Agents A1 Carro-Huerga, Guzmán A1 Mayo Prieto, Sara A1 Rodríguez González, Álvaro A1 González López, Óscar A1 Gutiérrez Martín, Santiago A1 Casquero Luelmo, Pedro Antonio A2 Produccion Vegetal K1 Ingeniería agrícola K1 Biological control K1 Grapevine trunk diseases K1 Viticulture K1 Sustainability K1 Phaeoacremonium minimum AB [EN] Fungi from the genus Trichoderma have a worldwide distribution and are commonly foundin agricultural lands. Further, it has been described as a non-virulent and symbiont microorganismthat can contribute to minimize the pernicious effects of pathogens. In the present work we have isolatedTrichoderma spp. from bark of grapevine in different orchards in order to determine the influenceof fungicide application and vine age on Trichoderma diversity in plant. An opposite correlation betweenthe number of fungicides sprayed per campaign and the diversity of Trichoderma spp. isolateswas found. Moreover, the older are grapevine plants the higher is the diversity of Trichoderma spp.isolates. The different Trichoderma strains isolated were tested against Phaeoacremonium minimum, agrapevine trunk pathogen, to evaluate their biocontrol capacity. Three Trichoderma strains shown asignificant capacity to control P. minimum and were selected as candidates to be used as biologicalcontrol agents. In addition, a rapid and easy method for isolating Trichoderma spp. from grapevineplants has been developed, which allowed to determine that the reduction in the amount of pesticideuse, together with the preservation of old vineyards, lead to healthier agroecosystems containinghigher levels of beneficial microorganisms PB MDPI LK https://hdl.handle.net/10612/21226 UL https://hdl.handle.net/10612/21226 NO Carro-Huerga, G., Mayo-Prieto, S., Rodríguez-González, Á., González-López, Ó., Gutiérrez, S., & Casquero, P. A. (2021). Influence of fungicide application and vine age on trichoderma diversity as source of biological control agents. Agronomy, 11(3). https://doi.org/10.3390/AGRONOMY11030446 DS BULERIA. Repositorio Institucional de la Universidad de León RD Jul 12, 2024