RT info:eu-repo/semantics/article
T1 Detection of putative peptide synthetase genes inTrichodermaspecies: Application of this method to the cloning of a gene fromT. harzianumCECT 2413
A1 Vizcaíno, Juan Antonio
A1 Sanz Andreu, Luis
A1 Cardoza, Rosa E.
A1 Monte Vázquez, Enrique
A1 Gutiérrez Martín, Santiago
A2 Microbiologia
K1 Biología
K1 Trichoderma
K1 Non-ribosomal peptide synthetase
K1 Degenerated-PCR
K1 Antibiosis
K1 Pseudogene
AB [EN] Some of the secondary metabolites produced by Trichoderma, such as the peptaibols and other antibiotics, have a peptide structure and in their biosynthesis are involved proteins belonging to the Non-Ribosomal Peptide Synthetase family. In the present work, a PCR-mediated strategy was used to clone a region corresponding to an adenylation domain of a peptide synthetase (PS) gene from 10 different strains of Trichoderma. In addition, and using the fragment isolated by PCR from T. harzianum CECT 2413 as a probe, a fragment of 19.0 kb corresponding to a PS-encoding gene named salps1, including a 1.5 kb fragment of the promoter, was cloned and sequenced. The cloned region of salps1 contains four complete, and a fifth incomplete, modules, in which are found the adenylation, thiolation and condensation domains, but also an additional epimerization domain at the C-terminal end of the first module. The analysis of the Salps1 protein sequence, taking into consideration published data, suggests that it is neither a peptaibol synthetase nor a protein involved in siderophore biosynthesis. The presence of two breaks in the open reading frame and the expression of this gene under nitrogen starvation conditions suggest that salps1 could be a pseudogene. © 2005 Federation of European Microbiological Societies
PB Oxford University Press
SN 0378-1097
LK https://hdl.handle.net/10612/21684
UL https://hdl.handle.net/10612/21684
NO Vizcaíno, Sanz, Cardoza, Monte, & Gutiérrez. (2005). Detection of putative peptide synthetase genes in Trichoderma species: Application of this method to the cloning of a gene from T. harzianum CECT 2413. FEMS Microbiology Letters, 244(1), 139-148. https://doi.org/10.1016/J.FEMSLE.2005.01.036
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 5, 2024