RT info:eu-repo/semantics/article
T1 Culture of Differentiated Adult Rabbit Auricular Chondrocytes
A1 Villar Suárez, María Vega
A1 Colaço, Bruno Jorge Antunes
A1 Calles-Venal, I.
A1 Fernández-Álvarez, G.
A1 Fernández-Caso, M.
A1 Villar-Lacilla, José María
A2 Otros
K1 Anatomía
K1 Veterinaria
AB Chondrocytes dedifferentiate to a fibroblast-like phenotypeon plastic surfaces. Dedifferentiation is reversible if thesecells are then cultured embedded in gels as alginate, agaroseor collagen. Chondrocytes cultured in suspension on a nonadherentsurface are also known to form aggregates ofdifferentiated cells. The knowledge of chondrocyte behaviorin culture is relevant for tissue engineering purposes. Inthis report we describe a simple method to culture differentiatedor redifferentiated rabbit auricular chondrocyteson plastic surfaces with a stable phenotype. When chondrocyteaggregates formed in suspension are next seeded onplastic surfaces, most of them attach to the plastic as roundor polygonal cells, and this morphological differentiation,confirmed by the presence of type II collagen, is stable forlong culture periods. We also report that the addition ofaggregates to monolayer cultures of dedifferentiatedchondrocytes results in their redifferentiation, as is shownby their morphological changes and the synthesis of type IIcollagen. Therefore, this simple method can be useful forthe study of chondrocyte behavior on plastic surfaces andfor redifferentiating previously proliferated chondrocytesin tissue engineering techniques. Furthermore, these resultsdemonstrate that, in addition to culture conditions such ascell isolation method or cell-density, chondrocyte behavioron plastic depends on the presence or absence of aggregatesresulting from the dissociation process
PB Asociación Mundial de Anatomistas Veterinarios
YR 2017
FD 2017-07-25
LK http://hdl.handle.net/10612/6450
UL http://hdl.handle.net/10612/6450
NO Anatomia, Histologia, Embryologia, December 2005,  Volume 34, Issue Supplement s1
NO P. 54
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024