RT info:eu-repo/semantics/article T1 Distribution of Tetracycline Resistance Genes in Actinobacillus pleuropneumoniae Isolates from Spain A1 Blanco, Mónica A1 Gutiérrez Martín, César Bernardo A1 Rodríguez Ferri, Elías Fernando A1 Roberts, Marilyn C. A1 Navas, Jesús A2 Sanidad Animal K1 Sanidad animal K1 Spain K1 Tetracycline K1 Actinobacillus pleuropneumoniae AB Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia. Tetracycline is used fortherapy of this disease, and A. pleuropneumoniae carrying the tet(B) gene, coding for an efflux protein thatreduces the intercellular tetracycline level, has been described previously. Of the 46 tetracycline-resistant (Tcr)Spanish A. pleuropneumoniae isolates used in this study, 32 (70%) carried the tet(B) gene, and 30 of these geneswere associated with plasmids. Eight (17%) isolates carried the tet(O) gene, two (4%) isolates carried either thetet(H) or the tet(L) gene, and all these genes were associated with plasmids. This is the first description of thesetet genes in A. pleuropneumoniae. The last two Tcr isolates carried none of the tet genes examined. Exceptfor tet(O)-containing plasmids, the other 34 Tcr plasmids were transformable into an Escherichia coli recipient.Two plasmids were completely sequenced. Plasmid p11745, carrying the tet(B) gene, was 5,486 bp and includeda rep gene, encoding a replication-related protein, and two open reading frames (ORFs) with homology tomobilization genes of Neisseria gonorrhoeae plasmid pSJ7.4. Plasmid p9555, carrying the tet(L) gene, was 5,672bp and, based on its G C content, consisted of two regions, one of putative gram-positive origin containing thetet(L) gene and the other comprising four ORFs organized in an operon-like structure with homology tomobilization genes in other plasmids of gram-negative bacteria PB American Society for Microbiology YR 2017 FD 2017-07-26 LK http://hdl.handle.net/10612/6451 UL http://hdl.handle.net/10612/6451 NO Antimicrobial Agents and Chemotherapy, febrero 2006, vol. 50, n. 2 NO P. 702-708 DS BULERIA. Repositorio Institucional de la Universidad de León RD 29-mar-2024