RT info:eu-repo/semantics/article
T1 Identification and characterization of the TonB region and its role in transferrin-mediated iron acquisition in Haemophilus parasuis
A1 Río González, María Luisa del
A1 Gutiérrez Martín, César Bernardo
A1 Rodríguez Barbosa, José Ignacio
A1 Navas, Jesús
A1 Rodríguez Ferri, Elías Fernando
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Haemophilus parasuis
K1 Iron acquisition
K1 TonB region
K1 Transferrin-binding protein
AB Haemophilus parasuis is the causative agent of Glässer's disease, which is responsible for considerable economic losses in the pigrearingindustry. The aim of the study reported here was the identification, sequencing and molecular characterization of the TonBregion that includes tonB, exbBD, and tbpBA genes in H. parasuis. In addition, two fusion proteins were generated. One of them(pGEX-6P-1-GST-TbpB) contained the first 501 amino acids of H. parasuis TbpB protein, while the second (pBAD-Thio-TbpBV5-His) included the first 102 amino acids of H. parasuis TbpB N-terminus domain. A panel of 14 hybridomas secreting monoclonalantibodies was raised against the two recombinant TbpB fusion proteins. Furthermore, to assess whether the expression of theH. parasuis ExbB, TbpB, and TbpA proteins was upregulated under conditions of restricted availability of iron, a rabbit polyclonalantibody against H. parasuis TbpB-His fusion protein was produced. A rabbit polyclonal antibody against serotype 7 of Actinobacilluspleuropneumoniae ExbB and TbpA proteins was also used for the detection of the homologous proteins in H. parasuis. Overall,the data indicate that H. parasuis, like other members of the Pasteurellaceae family, possesses the genetic elements of the TonBregion for iron acquisition and the transferrin-binding proteins encoded under this region are upregulated under restricted ironavailability
PB Elsevier
YR 2017
FD 2017-08-02
LK http://hdl.handle.net/10612/6470
UL http://hdl.handle.net/10612/6470
NO FEMS Immunology and Medical Microbiology, 2005
NO 12 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024