RT info:eu-repo/semantics/article T1 Acute phase protein concentrations in colostrum-deprived pigs immunized with subunit and commercial vaccines against Glässer’s disease A1 Martínez Martínez, Sonia A1 Frandoloso, Rafael A1 Gutiérrez Martín, César Bernardo A1 Lampreave, Fermín A1 García Iglesias, María José A1 Pérez Martínez, Claudia A1 Rodríguez Ferri, Elías Fernando A2 Sanidad Animal K1 Sanidad animal K1 Veterinaria K1 Acute-phase proteins K1 Haemophilus parasuis K1 Glässer’s disease AB Haemophilus parasuis is the etiological agent of Glässer’s disease, which is characterizedby fibrinous polyserositis, polyarthritis and meningitis in pigs. This study was focusedon the characterization of the acute-phase response after immunization and infection ofcolostrum-deprived pigs with H. parasuis serovar 5, by measuring serum concentrations ofthree positive acute-phase proteins (APPs) (pig major acute-phase protein pig, MAP; haptoglobin,HPG; C-reactive protein, CRP) and one negative APP (apolipoprotein A-I, ApoA-I).Six experimental groups were established: a non-immunized but infected control group(CTL); two groups immunized with either a recombinant transferrin-binding protein (Tbp)A or TbpB fragment from H. parasuis Nagasaki strain (rTbpA and rTbpB, respectively); twogroups immunized with native outer membrane proteins with affinity to porcine transferrin(NPAPT), one of them inoculated intramuscularly (NPAPTim) and the other intratracheally(NPAPTit), and the last group receiving a commercially available bacterin (PG). The greatestconcentrations of the three positive APPs and the lowest concentration of the negative APPwere detected in CTL group, as well as in those animals belonging to rTbpA or rTbpB groupsthat died in response to challenge. Significant differences (P < 0.005) were found in thesegroups when comparing challenge with the following days after it. However, no significantdifferences were seen for the remaining vaccinated groups (NPAPTim, NPAPTit and PG),which were effectively protected against Glässer’s disease. Therefore, APPs could be usedas useful biomarkers for both evaluating disease progression and determining vaccinationeffectiveness PB Elsevier YR 2017 FD 2017-08-02 LK http://hdl.handle.net/10612/6471 UL http://hdl.handle.net/10612/6471 NO Veterinary Immunology and Immunopathology, 2011 NO 8 p. DS BULERIA. Repositorio Institucional de la Universidad de León RD 29-mar-2024