RT info:eu-repo/semantics/article
T1 Haemophilus parasuis serovar 5 Nagasaki strain adheres and invades PK-15 cells
A1 Frandoloso, Rafael
A1 Martínez Martínez, Sonia
A1 Gutiérrez Martín, César Bernardo
A1 Rodríguez Ferri, Elías Fernando
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Haemophilus parasuis
K1 Glässer’s disease
K1 Adherence
K1 Invasion
AB Haemophilus parasuis is the agent responsible for causing Gla¨ sser’s disease, which ischaracterized by fibrinous polyserositis, polyarthritis and meningitis in pigs. The purposeof this study was to investigate the in vitro ability of two H. parasuis serovars of differentvirulence (serovar 5, Nagasaki strain, highly virulent, belonging to serovar 5, and SW114strain, nonvirulent, belonging to serovar 3) to adhere to and invade porcine kidneyepithelial cells (PK-15 line). Nagasaki strain was able to attach at high levels from 60 to180 min of incubation irrespective of the concentrations compared (107–1010 CFU), and asubstantial increase of surface projections could be seen in PK-15 cells by scanningelectron microscopy. This virulent strain was also able to invade effectively theseepithelial cells, and the highest invasion capacity was reached at 180 min of infection. Onthe contrary, nonvirulent SW114 strain hardly adhered to PK-15 cells, and it did not invadethese cells, thus suggesting that adherence and invasion of porcine kidney epithelial cellscould be a virulence mechanism involved in the lesions caused by H. parasuis Nagasakistrain in this organ
PB Elsevier
YR 2017
FD 2017-08-02
LK http://hdl.handle.net/10612/6472
UL http://hdl.handle.net/10612/6472
NO Veterinary Microbiology, 2011
NO 7 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-abr-2024