RT info:eu-repo/semantics/article
T1 Molecular characterization of Haemophilus parasuis ferric hydroxamate uptake (fhu) genes and constitutive expression of the FhuA receptor
A1 Río González, María Luisa del
A1 Navas, Jesús
A1 Martín, Ana Judith
A1 Gutiérrez Martín, César Bernardo
A1 Rodríguez Barbosa, José Ignacio
A1 Rodríguez Ferri, Elías Fernando
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Haemophilus parasuis
K1 Ferric hydroxamate uptake
K1 Iron
AB Bacteria have evolved a set of highly specialized proteins to capture iron in irondepletedenvironments. The acquisition and uptake of iron present in the extracellular milieu ofeukaryotic organisms is indispensable for the growth and survival of microbial pathogens in thecourse of infection. Haemophilus parasuis is the causative agent of Glässer disease, which isresponsible for considerable financial losses in pig-rearing worldwide. To gain insight into themechanisms involved in siderophore-mediated iron uptake in H. parasuis, genes in the H. parasuisferric hydroxamate uptake (Fhu) region were amplified in the work being reported here. As has beendescribed in A. pleuropneumoniae, an Fhu genomic region was also present in H. parasuis, beingcomposed of four potential consecutive open reading frames (ORF) designated as fhuC, fhuD, fhuB,and fhuA, respectively. By immunoblotting, using a cross-reactive polyclonal antibody raisedagainst Actinobacillus pleuropneumoniae FhuA protein, it was demonstrated that this protein wasconstitutively expressed in H. parasuis and its level of expression was not modified underconditions of restricted iron availability. This is the first report describing the presence of the fhugenes in H. parasuis. Our results indicate that FhuA protein expression is not affected under ironrestrictedconditions, however, it is one of the targets of the humoral immune response
PB BioMed Central
YR 2017
FD 2017-08-23
LK http://hdl.handle.net/10612/6686
UL http://hdl.handle.net/10612/6686
NO Veterinary Research, 2006, vol. 37
NO P. 49-59
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 18-abr-2024