RT info:eu-repo/semantics/article
T1 A vaccine based on a mutant transferrin binding protein B of Haemophilus parasuis induces a strong T-helper 2 response and bacterial clearance after experimental infection
A1 Martínez Martínez, Sonia
A1 Frandoloso, Rafael
A1 Rodríguez Ferri, Elías Fernando
A1 García Iglesias, María José
A1 Pérez Martínez, Claudia
A1 Álvarez Estrada, Álvaro
A1 Gutiérrez Martín, César Bernardo
A2 Sanidad Animal
K1 Sanidad animal
K1 Haemophilus parasuis
K1 Glässer's disease
K1 Mutant TbpB vaccine
K1 Bacterias
K1 Antibodies
K1 Cytokines
AB This study aimed to characterize the type of immune response induced by an experimental vaccine based on a mutantHaemophilus parasuis transferrin binding protein (Tbp) B (Y167A) defective in its ability to bind porcine transferrin.Clinical and pathological signs, bacterial clearance, antibody response and the cytokine profile in alveolar macrophagesand spleen after the vaccination and challenge of twenty-two colostrum-deprived pigs with 108 CFU of H. parasuis wereanalysed. Pigs vaccinated with Y167A were compared to those vaccinated with native TbpB (nTbpB), those treated witha commercial bacterin (CB) against Glässer's disease, those unvaccinated challenged (CH) and those unvaccinated unchallenged(UNCH) pigs. The rectal temperatures of Y167A pigs resembled those of UNCH pigs and were significantlylower than those of the nTbpB, CB and CH animals. A major reduction in pathological changes of the challenged pigswas observed in the Y167A group. H. parasuis was cleared from 88.9% of the samples from Y167A pigs versus 60.0%and 55.6% from those of the CB and nTbpB groups, respectively. The antibody response elicited by Y167A by ELISAwas notably higher than that observed for nTbpB and CB pigs and was capable of preventing the expression and secretionof IL-8. The expression of IL-4 and IL-5, which were associated with the specific antibody levels, suggests that the mainmechanism of protection conferred by Y167A vaccine is based on a strong T-helper 2 response
PB Elsevier
YR 2017
FD 2017-09-28
LK http://hdl.handle.net/10612/6816
UL http://hdl.handle.net/10612/6816
NO Veterinary Immunology and Immunopathology, 2016
NO 9 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024